Morel A, Darmon M, Delaage M
Immunotech, Marseille, France.
Mol Immunol. 1990 Oct;27(10):995-1000. doi: 10.1016/0161-5890(90)90122-g.
The different ways of raising antibodies to histamine are reviewed. High affinity monoclonal antibodies could be raised only against derivatized histamine. Succinyl glycinamide derivatization provided the basis of an efficient radioimmunoassay. In this paper the molecular pattern and the thermodynamical properties of histamine recognition were thoroughly investigated. Only the neutral form and not the cationic form of imidazole was recognized. As expected, the ligand recognition increased, with improved structural homology to the immunogen. However, a detailed analysis revealed a zwitterionic effect whenever a carboxylic group was present on the side chain of the ligand.
本文综述了制备组胺抗体的不同方法。高亲和力单克隆抗体只能针对衍生化的组胺制备。琥珀酰甘氨酰胺衍生化为高效放射免疫分析提供了基础。本文对组胺识别的分子模式和热力学性质进行了深入研究。仅识别咪唑的中性形式而非阳离子形式。正如预期的那样，随着与免疫原的结构同源性提高，配体识别能力增强。然而，详细分析表明，只要配体侧链上存在羧基，就会产生两性离子效应。
