Mita H, Yasueda H, Shida T, Baba S
Agents Actions. 1984 Jun;14(5-6):574-9. doi: 10.1007/BF01978889.
Three conjugates of different histamine derivatives to bovine serum albumin were prepared, and an attempt was made to determine whether antibody against histamine and histamine derivatives could be produced in rabbits by immunization with these conjugates. Antibody produced by immunization with bovine serum albumin-succinylhistamine conjugate could not recognize the hapten moiety of the immunogen. Immunization of rabbits with conjugate of bovine serum albumin and p-[2-(N alpha-trifluoroacetylhistamine)azo]benzoic acid resulted in the production of antibody to N alpha-trifluoroacetylhistamine. The antibody had a specificity for histamine metabolites and their derivatives. However, the antibody also showed a small but not negligible affinity for trifluoroacetylated serotonin, norepinephrine and aniline, indicating that the antibody specificity was directed mainly to the trifluoroacetamide group. After immunization of rabbits with bovine serum albumin and p-[2-(N alpha-propionylhistamine)azo]benzoic acid conjugate, antibody against N alpha-propionylhistamine could be produced. However, the antibody had a very low affinity even for N alpha-propionylhistamine. Specific antibody to histamine and histamine derivatives could thus not be produced by immunization with the present hapten-carrier conjugates.
制备了三种不同组胺衍生物与牛血清白蛋白的缀合物，并尝试确定用这些缀合物免疫兔子是否能产生针对组胺和组胺衍生物的抗体。用牛血清白蛋白 - 琥珀酰组胺缀合物免疫产生的抗体无法识别免疫原的半抗原部分。用牛血清白蛋白与对 - [2 - (Nα - 三氟乙酰组胺)偶氮]苯甲酸的缀合物免疫兔子，产生了针对Nα - 三氟乙酰组胺的抗体。该抗体对组胺代谢物及其衍生物具有特异性。然而，该抗体对三氟乙酰化的血清素、去甲肾上腺素和苯胺也表现出小但不可忽略的亲和力，表明抗体特异性主要针对三氟乙酰胺基团。用牛血清白蛋白与对 - [2 - (Nα - 丙酰组胺)偶氮]苯甲酸缀合物免疫兔子后，可产生针对Nα - 丙酰组胺的抗体。然而，该抗体对Nα - 丙酰组胺的亲和力非常低。因此，用目前的半抗原 - 载体缀合物免疫无法产生针对组胺和组胺衍生物的特异性抗体。
