Consortium for Translational Orthodontic Research, New York University College of Dentistry, New York, NY 10010, USA.
J Dent Res. 2012 Apr;91(4):413-9. doi: 10.1177/0022034512438590. Epub 2012 Feb 14.
Mechanical stimulation contributes to the health of alveolar bone, but no therapy using the osteogenic effects of these stimuli to increase alveolar bone formation has been developed. We propose that the application of high-frequency acceleration to teeth in the absence of significant loading is osteogenic. Sprague-Dawley rats were divided among control, sham, and experimental groups. The experimental group underwent localized accelerations at different frequencies for 5 min/day on the occlusal surface of the maxillary right first molar at a very low magnitude of loading (4 µε). Sham rats received a similar load in the absence of acceleration or frequency. The alveolar bone of the maxilla was evaluated by microcomputed tomography (µCT), histology, fluorescence microscopy, scanning electron microscopy (SEM), Fourier Transform Infrared Spectroscopy (FTIR imaging), and RT-PCR for osteogenic genes. Results demonstrate that application of high-frequency acceleration significantly increased alveolar bone formation. These effects were not restricted to the area of application, and loading could be replaced by frequency and acceleration. These studies propose a simple mechanical therapy that may play a significant role in alveolar bone formation and maintenance.
机械刺激有助于肺泡骨的健康，但尚未开发出利用这些刺激的成骨作用来增加肺泡骨形成的治疗方法。我们提出，在没有明显负荷的情况下，应用高频加速度对牙齿具有成骨作用。将 Sprague-Dawley 大鼠分为对照组、假手术组和实验组。实验组在加载非常小（4µε）的情况下，每天在右上颌第一磨牙的咬合面上接受不同频率的局部加速 5 分钟。假手术组在没有加速或频率的情况下接受类似的负荷。通过 microCT（µCT）、组织学、荧光显微镜、扫描电子显微镜（SEM）、傅里叶变换红外光谱（FTIR 成像）和 RT-PCR 对骨生成基因评估上颌牙槽骨。结果表明，应用高频加速度可显著增加牙槽骨形成。这些效果不仅限于应用区域，并且可以用频率和加速度代替负载。这些研究提出了一种简单的机械治疗方法，可能在牙槽骨形成和维持中发挥重要作用。
