Judex Stefan, Pongkitwitoon Suphannee
Integrative Skeletal Adaptation and Genetics Laboratory, Department of Biomedical Engineering, Stony Brook University, NY, USA.
Department of Orthopaedic Surgery, Columbia University, NY, USA.
Dose Response. 2018 Aug 16;16(3):1559325818792112. doi: 10.1177/1559325818792112. eCollection 2018 Jul-Sep.
Modalities that increase the rate of tooth movement have received considerable attention, but direct comparisons between devices are rare. Here, we contrasted 2 mechanical vibratory devices designed to directly transfer vibrations into alveolar bone as a means to influence bone remodeling. To this end, 3 cells types intimately involved in modulating tooth movements-osteoblasts, periodontal ligament fibroblasts, and osteoclasts-were subjected to in vitro vibrations at bout durations prescribed by the manufacturers. As quantified by an accelerometer, vibration frequency and peak accelerations were 400% and 70% greater in the VPro5 (Propel Orthodontics) than in the AcceleDent (OrthoAccel Technologies) device. Both devices caused increased cell proliferation and gene expression in osteoblasts and fibroblasts, but the response to VPro5 treatment was greater than for the AcceleDent. In contrast, the ability to increase osteoclast activity was device independent. These data present an important first step in determining how specific cell types important for facilitating tooth movement respond to different vibration profiles. The device that engendered a higher vibration frequency and larger acceleration (VPro5) was superior in stimulating osteoblast and fibroblast cell proliferation/gene expression, although the duration of each treatment bout was 75% shorter than for the AcceleDent.
能够提高牙齿移动速率的方法已受到广泛关注，但不同设备之间的直接比较却很少见。在此，我们对比了两种旨在将振动直接传递至牙槽骨以影响骨重塑的机械振动设备。为此，我们让与调节牙齿移动密切相关的三种细胞类型——成骨细胞、牙周膜成纤维细胞和破骨细胞——在制造商规定的发作持续时间内接受体外振动。通过加速度计量化后发现，VPro5（Propel Orthodontics公司）的振动频率和峰值加速度比AcceleDent（OrthoAccel Technologies公司）设备分别高400%和70%。两种设备均能使成骨细胞和成纤维细胞的细胞增殖及基因表达增加，但VPro5处理后的反应比AcceleDent更强烈。相比之下，提高破骨细胞活性的能力与设备无关。这些数据为确定促进牙齿移动的特定细胞类型如何对不同振动模式作出反应迈出了重要的第一步。产生更高振动频率和更大加速度的设备（VPro5）在刺激成骨细胞和成纤维细胞增殖/基因表达方面更具优势，尽管每次处理发作的持续时间比AcceleDent短75%。
