[辅助性T细胞17在免疫性血小板减少症小鼠模型中的作用]

[The role of Th17 cells in immune thrombocytopenia mice models].

作者信息

Wang Ting-ting, Li Hui-yuan, Wang Zhao, Zhou Ze-ping, Fu Li, Wei Na, Huang Da-yong, Yang Ren-chi

机构信息

Department of Hematology, Beijing Friendship Hospital, Capital Medical University, Beijing 100050, China.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 2011 Sep;32(9):592-6.

PMID:22338151

Abstract

OBJECTIVE

To investigate the role of Th17 cells in immune thrombocytopenia (ITP) mice model.

METHOD

ITP was induced by daily intraperitoneal injection of anti-platelet membrane CD41 antibody (MWReg30) into BALB/c mice, the mRNA expressions of Th17 cell associated transcription factors and cytokines in peripheral blood and spleen mononuclear cells were measured by real-time PCR, and the proportion of Th17 cells by FCM analysis.

RESULTS

The percentage of Th17 cell was significantly elevated in ITP mice both in splenocyte and peripheral blood as compared with that in normal controls (P<0.01). ITP mice had elevated mRNA expressions of IL-17F, IL-17A and IL-6 in splenocyte (P<0.05), and of IL-21 in peripheral blood (P<0.05). There was a positive correlation between IL-17A and IL-17F (r = 0.934, P = 0.000), and between IL-17A/IL-17F and IL-6 (r = 0.598, P = 0.001; r = 0. 619, P = 0.000).

CONCLUSIONS

Th17 cell might play an important role in the pathogenesis of ITP, at least involving in the clearance of platelets.

摘要

目的

探讨Th17细胞在免疫性血小板减少症（ITP）小鼠模型中的作用。

方法

通过每日腹腔注射抗血小板膜CD41抗体（MWReg30）诱导BALB/c小鼠发生ITP，采用实时PCR检测外周血和脾脏单个核细胞中Th17细胞相关转录因子及细胞因子的mRNA表达，并用流式细胞术分析Th17细胞比例。

结果

与正常对照组相比，ITP小鼠脾脏细胞和外周血中Th17细胞百分比显著升高（P<0.01）。ITP小鼠脾脏细胞中IL-17F、IL-17A和IL-6的mRNA表达升高（P<0.05），外周血中IL-21的mRNA表达升高（P<0.05）。IL-17A与IL-17F之间呈正相关（r = 0.934，P = 0.000），IL-17A/IL-17F与IL-6之间也呈正相关（r = 0.598，P = 0.001；r = 0.619，P = 0.000）。