[CD8+效应性T细胞对重型再生障碍性贫血患者造血途径损伤的影响]

[Effect of CD8+ effector T cells on the hematopoiesis pathway damage in the patients with severe aplastic anemia].

作者信息

Feng Le, Fu Rong, Wang Hua-quan, Wang Jun, Liu Chun-yan, Li Li-juan, Liu Hui, Wang Hong-lei, Zhang Tian, Ruan Er-bao, Liang Yong, Qu Wen, Wang Guo-jin, Wu Yu-hong, Liu Hong, Wang Xiao-ming, Song Jia, Guan Jing, Xing Li-min, Shao Zong-hong

机构信息

Department of Hematology & Oncology,Tianjin Medical University General Hospital, Tianjin 300052, China.

出版信息

Zhonghua Xue Ye Xue Za Zhi. 2011 Sep;32(9):597-601.

PMID:22338152

Abstract

OBJECTIVE

To investigate the quantity and the pathway to damage hematopoietic cells of CD8+CD25+ and CD8+ HLA-DR+ effector T cells in peripheral blood (PB) of severe aplastic anemia(SAA) patients and explore the immunopathogenesis of SAA.

METHODS

The quantity of CD8+ CD25+ and CD8+ HLA-DR+ cells in PB and the expressions of perforin, granzyme B, tumor necrosis factor-beta (TNF-beta) and FasL in 29 SAA (14 untreated and 15 recovered) patients and 12 normal controls were analyzed by flow cytometry.

RESULTS

The fraction of CD8+ CD25+ T cells in CD8+ T cells was (3.67 +/- 2.58)% in untreated SAA patients, (5.19 +/- 4. 29)% in recovered patients and (4.84 +/- 2.31)% in normal controls, and that of CD8+ CD25+ T cells in CD3+ cells in the three groups was (2.25 +/- 1.35)%, (2.98 +/- 1.35)% and (2.11 +/- 1.88)%, respectively. They had no statistic difference among the 3 groups (P >0.05). The fraction of CD8+ HLA-DR+ T cells in CD8+ T cells was (39.30 +/- 8.13)% in untreated patients, which was significantly higher than that in recovered patients [(20.65 +/- 5.38)%] and controls [(18.34 +/- 6.68)%] (P<0.001), while there was no statistic difference between the latter two groups (P>0.05). CD8+ HLA-DR+ T cells in CD3+ cells was (27.81 +/- 7.10)% in untreated group, which was significantly higher than that of recovered group [(12.02 +/- 3.03)%] and controls [(8.50 +/-2.33)%] (P<0.01). And that in recovered group was higher than that in control group (P<0.05). The expressions of perforin, granzyme B, TNF-beta and FasL of CD8+ HLA-DR+ T cells in untreated group were 8.51%, 96.08%, 72.11% and 94.25% respectively, which were higher than those in recovered group (1.78%, 85.20%, 34.38% and 51.20%) and controls (1.86%, 82.09% ,17.92% and 32.91%). There was no statistic difference between recovered patients and controls (P>0.05).

CONCLUSION

There were elevated quantity of CD8+ HLA-DR+ T cells and high expressions of perforin, granzyme B, TNF-beta and FasL in SAA, which might contribute to the bone marrow failure.

摘要

目的

探讨重型再生障碍性贫血（SAA）患者外周血中CD8+CD25+及CD8+HLA-DR+效应性T细胞对造血细胞的损伤数量及途径，以探讨SAA的免疫发病机制。

方法

采用流式细胞术分析29例SAA患者（14例未治疗、15例已缓解）及12名正常对照者外周血中CD8+CD25+及CD8+HLA-DR+细胞数量，以及穿孔素、颗粒酶B、肿瘤坏死因子-β（TNF-β）和FasL的表达。

结果

未治疗SAA患者CD8+T细胞中CD8+CD25+T细胞比例为（3.67±2.58）%，缓解患者为（5.19±4.29）%，正常对照者为（4.84±2.31）%；三组CD3+细胞中CD8+CD25+T细胞比例分别为（2.25±1.35）%、（2.98±1.35）%和（2.11±1.88）%。三组间差异无统计学意义（P>0.05）。未治疗患者CD8+T细胞中CD8+HLA-DR+T细胞比例为（39.30±8.13）%，显著高于缓解患者[（20.65±5.38）%]及对照者[（18.34±6.68）%]（P<0.001），而后两组间差异无统计学意义（P>0.05）。未治疗组CD3+细胞中CD8+HLA-DR+T细胞比例为（27.81±7.10）%，显著高于缓解组[（12.02±3.03）%]及对照者[（8.50±2.33）%]（P<0.01），缓解组高于对照组（P<0.05）。未治疗组CD8+HLA-DR+T细胞穿孔素、颗粒酶B、TNF-β和FasL表达分别为8.51%、96.08%、72.11%和94.25%，高于缓解组（1.78%、85.20%、34.38%和51.20%）及对照者（1.86%、82.09%、17.92%和32.91%）。缓解患者与对照者间差异无统计学意义（P>0.05）。