Therapeutical Chemistry Department, National Research Center, Giza, Egypt.
Eur Rev Med Pharmacol Sci. 2012 Jan;16(1):66-78.
It is well documented that oxidative stress is a basic mechanism behind the development of diabetic state. The current study was undertaken to elucidate the hypoglycemic role of zinc, selenium and vitamin E and their mixture in comparison with the antidiabetic drug glibenclamide.
Male Wistar rats weighing 250 +/- 50 g were made diabetic by injection with a single i.p. dose of streptozotocin (STZ) (65 mg/kg b. wt). Diabetic groups were simultaneously i.p. injected either with zinc chloride (ZnCI2) (5 mg/kg) or with selenium and vitamin E (1.5 mg/kg as sodium selenite and vitamin E 1000 mg/kg) or with zinc, selenium and vitamin E each element i.p. injected according to its corresponding therapeutic dose daily for one month. Another group was orally treated daily with glibenclamide drug (5 mg/kg) for one month.
Blood and tissue samples were collected at day 3 post STZ injection (from one group serum glucose level significantly elevated < or = 300, p < or = 0.05) and at day 30 post-treatment in other groups. Liver function, nitric oxide (NO), malondialdehyde (MDA) and phosphoenol pyruvate carboxykinase (PEPCK) were significantly increased, while superoxide dismutase (SOD), reduced glutathione (GSH), total protein, lactate dehydrogenase (LDH), pyruvate kinase (PK) and hexokinase (HK) were inhibited after STZ treatment. Histological examination of diabetic liver showed necrosis and degenerative changes of hepatocytes. Treatment of diabetic rats with ZnCI2, selenium and vitamin E or their combination blunted the increment in serum glucose induced by STZ, preserved liver architecture and ameliorated all the previous mentioned biochemical parameters.
It was found that, the combined administration of zinc, selenium and vitamin E exhibited a more remarkable effect than either zinc or selenium and vitamin E. So, the results clearly indicate the beneficial effects of micronutrients combination in controlling hyperglycemia.
氧化应激是糖尿病发展的基本机制，这一点已得到充分证实。本研究旨在阐明锌、硒和维生素 E 及其混合物的降血糖作用，并将其与抗糖尿病药物格列本脲进行比较。
雄性 Wistar 大鼠体重 250 ± 50 g，经单次腹腔注射链脲佐菌素（STZ）（65 mg/kg 体重）诱导糖尿病。糖尿病组同时腹腔注射氯化锌（ZnCI2）（5 mg/kg）或硒和维生素 E（1.5 mg/kg 作为亚硒酸钠和维生素 E 1000 mg/kg）或锌、硒和维生素 E 每个元素根据其相应的治疗剂量每天腹腔注射一次，共一个月。另一组每天口服格列本脲药物（5 mg/kg）治疗一个月。
在 STZ 注射后第 3 天（一组血清葡萄糖水平显著升高≤300，p≤0.05）和第 30 天收集血液和组织样本。在其他组中。肝功能、一氧化氮（NO）、丙二醛（MDA）和磷酸烯醇丙酮酸羧激酶（PEPCK）显著升高，而超氧化物歧化酶（SOD）、还原型谷胱甘肽（GSH）、总蛋白、乳酸脱氢酶（LDH）、丙酮酸激酶（PK）和己糖激酶（HK）在 STZ 处理后受到抑制。糖尿病肝组织学检查显示肝细胞坏死和变性。用 ZnCI2、硒和维生素 E 或其组合治疗糖尿病大鼠可减弱 STZ 引起的血清葡萄糖升高，维持肝结构，并改善所有上述生化参数。
研究发现，锌、硒和维生素 E 的联合给药比锌或硒和维生素 E 具有更显著的效果。因此，结果清楚地表明了微量元素组合在控制高血糖方面的有益作用。
