Synchrotron SOLEIL, L'Orme des Merisiers, 91192 Gif sur Yvette Cedex, France.
J Synchrotron Radiat. 2012 Mar;19(Pt 2):174-8. doi: 10.1107/S0909049512001057. Epub 2012 Jan 31.
A novel experimental technique for tandem mass spectrometry and ion spectroscopy of electrosprayed ions using vacuum-ultraviolet (VUV) synchrotron radiation is presented. Photon activation of trapped precursor ions has been performed by coupling a commercial linear quadrupole ion trap (Thermo scientific LTQ XL), equipped with the electrosprayed ions source, to the DESIRS beamline at the SOLEIL synchrotron radiation facility. The obtained results include, for the first time on biopolymers, photodetachment spectroscopy using monochromated synchrotron radiation of multi-charged anions and the single photon ionization of large charge-selected polycations. The high efficiency and signal-to-noise ratio achieved by the present set-up open up possibilities of using synchrotron light as a new controllable activation method in tandem mass spectrometry of biopolymers and VUV-photon spectroscopy of large biological ions.
提出了一种用于串联质谱和电喷雾离子离子光谱学的新型实验技术，使用真空紫外（VUV）同步加速器辐射。通过将配备电喷雾离子源的商业线性四极离子阱（Thermo scientific LTQ XL）与 SOLEIL 同步加速器辐射设施的 DESIRS 光束线耦合，实现了被捕获的前体离子的光活化。首次在生物聚合物上获得的结果包括使用同步加速器辐射的单色光对多电荷阴离子进行光离解谱和对大电荷选择的聚阳离子进行单光子电离。本装置实现的高效率和信噪比为使用同步加速器光作为生物聚合物串联质谱学和大生物离子 VUV 光子光谱学的新型可控活化方法开辟了可能性。
