Vural Burcak, Yakar Fatih, Derin Duygu, Saip Pınar, Yakar Aysun, Demirkan Ayşe, Karabulut Aydın, Ugurel Elif, Cine Naci, Kilicaslan Zeki, Tüzün Erdem, Ozbek Ugur
Department of Genetics, Institute for Experimental Medicine (DETAE), Istanbul University, Istanbul, Turkey.
Genet Test Mol Biomarkers. 2012 Jul;16(7):701-6. doi: 10.1089/gtmb.2011.0315. Epub 2012 Feb 17.
Glutathione S-transferase P1 (GSTP1) plays an important role in cellular protection against oxidative stress and toxic chemicals. Polymorphisms within GSTP1 are associated with alterations in enzyme activity, which may lead to development of lung disease and cancer. In this study, we aimed to investigate the GSTP1 Ile105Val and Ala114Val polymorphisms in patients with small cell lung cancer (SCLC).
PATIENTS/METHODS: GSTP1 Ile105Val polymorphism in exon 5 and GSTP1 Ala114Val polymorphism in exon 6 were determined by using polymerase chain reaction-restriction fragment length polymorphism techniques in 89 patients with SCLC and 108 control patients with chronic obstructive pulmonary disease (COPD). Genotype frequencies and cigarette smoking intensities were compared among SCLC and COPD patients.
There were significantly less SCLC patients with variant exon 6 genotypes than COPD patients (7.9% vs. 20.4%, p=0.007), while the number of patients with variant exon 5 genotypes were comparable among groups. SCLC and COPD patients with variant exon 6 genotype showed trends toward exhibiting reduced cigarette consumption.
The variant GSTP1 exon 6 genotype might be conferring protection against SCLC development. Whether this effect is associated with exposure to cigarette smoking needs to be clarified.
谷胱甘肽S-转移酶P1（GSTP1）在细胞抵御氧化应激和有毒化学物质的过程中发挥着重要作用。GSTP1基因多态性与酶活性的改变有关，这可能会导致肺部疾病和癌症的发生。在本研究中，我们旨在调查小细胞肺癌（SCLC）患者的GSTP1 Ile105Val和Ala114Val多态性。
患者/方法：采用聚合酶链反应-限制性片段长度多态性技术，对89例小细胞肺癌患者和108例慢性阻塞性肺疾病（COPD）对照患者进行第5外显子的GSTP1 Ile105Val多态性和第6外显子的GSTP1 Ala114Val多态性检测。比较小细胞肺癌患者和慢性阻塞性肺疾病患者的基因型频率和吸烟强度。
小细胞肺癌患者中第6外显子变异基因型的患者明显少于慢性阻塞性肺疾病患者（7.9%对20.4%，p=0.007），而第5外显子变异基因型的患者数量在两组间相当。第6外显子变异基因型的小细胞肺癌患者和慢性阻塞性肺疾病患者有吸烟量减少的趋势。
GSTP1第6外显子变异基因型可能对小细胞肺癌的发生具有保护作用。这种作用是否与吸烟暴露有关尚需进一步明确。
