Yonsei University College of Dentistry, Conservative Dentistry, Gangnam Severance Hospital, Seoul, Republic of Korea.
Oper Dent. 2012 Sep-Oct;37(5):532-9. doi: 10.2341/11-371-L. Epub 2012 Feb 16.
The purpose of this study was to investigate the effects of surface roughness of resin composite on biofilm formation of Streptococcus mutans in the presence of saliva. To provide uniform surface roughness on composites, disks were prepared by curing composite against 400-grit silicon carbide paper (SR400), 800-grit silicon carbide paper (SR800), or a glass slide (SRGlass). The surface roughness was examined using confocal laser microscopy. For biofilm formation, S. mutans was grown for 24 hours with each disk in a biofilm medium with either glucose or sucrose in the presence of fluid-phase or surface-adsorbed saliva. The adherent bacteria were quantified via enumeration of the total viable counts of bacteria. Biofilms were examined using scanning electron microscopy. This study showed that SR400 had deeper and larger, but fewer depressions than SR800. Compared to SRGlass and SR800, biofilm formation was significantly increased on SR400. In addition, the differences in the effect of surface roughness on the amount of biofilm formation were not significantly influenced by either the presence of saliva or the carbohydrate source. Considering that similar differences in surface roughness were observed between SR400 and SR800 and between SR800 and SRGlass, this study suggests that surface topography (size and depth of depressions) may play a more important role than surface roughness in biofilm formation of S. mutans .
本研究旨在探讨在唾液存在的情况下，树脂复合材料表面粗糙度对变形链球菌生物膜形成的影响。为了在复合材料上提供均匀的表面粗糙度，通过将复合材料固化在 400 目碳化硅砂纸（SR400）、800 目碳化硅砂纸（SR800）或玻璃载玻片（SRGlass）上来制备圆盘。使用共聚焦激光显微镜检查表面粗糙度。为了进行生物膜形成，将每种圆盘在含有葡萄糖或蔗糖的生物膜培养基中与流体相或表面吸附的唾液一起培养 24 小时。通过对细菌总数的计数来定量黏附细菌。使用扫描电子显微镜检查生物膜。本研究表明，SR400 比 SR800 具有更深和更大但更少的凹陷。与 SRGlass 和 SR800 相比，SR400 上的生物膜形成显著增加。此外，表面粗糙度对生物膜形成量的影响差异不受唾液或碳水化合物来源的影响。考虑到在 SR400 和 SR800 之间以及在 SR800 和 SRGlass 之间观察到相似的表面粗糙度差异，本研究表明，表面形貌（凹陷的大小和深度）可能比变形链球菌生物膜形成中的表面粗糙度更重要。
