Area of Molecular Biology and Biotechnology, ANFACO-CECOPESCA, Vigo, 36310 Pontevedra, Spain.
J Agric Food Chem. 2012 Feb 29;60(8):1893-7. doi: 10.1021/jf2043532. Epub 2012 Feb 16.
Crustaceans are one of the most common allergens causing severe food reaction. These food allergens are a health problem, and they have become very important; there are various regulations that establish that labeling must be present regarding these allergens to warn consumers. In the present work a fast real-time PCR, by a LNA probe, was developed. This allows the detection of crustaceans in all kinds of products, including processed products in which very aggressive treatments of temperature and pressure during the manufacturing process are used. This methodology provides greater sensitivity and specificity and reduces the analysis time of real-time PCR to 40 min. This methodology was further validated by means of simulating products likely to contain this allergen. For this, products present on the market were spiked with crustacean cooking water. The assay is a potential tool in issues related to the labeling of products and food security to protect the allergic consumer.
甲壳类动物是引起严重食物反应的最常见过敏原之一。这些食物过敏原是一个健康问题，已经变得非常重要；有各种规定要求对这些过敏原进行标签标注，以警告消费者。在本工作中，开发了一种快速实时 PCR 方法，通过 LNA 探针，可以检测各种产品中的甲壳类动物，包括在制造过程中使用非常高的温度和压力处理的加工产品。该方法提供了更高的灵敏度和特异性，并将实时 PCR 的分析时间缩短至 40 分钟。该方法通过模拟可能含有这种过敏原的产品进一步得到了验证。为此，在市场上的产品中加入了甲壳类动物烹饪水。该检测方法是与产品标签和食品安全相关的问题的潜在工具，可保护过敏消费者。
