一种用于检测复杂食品基质中蟹（甲壳类过敏原）的特定群体、定量实时 PCR 检测方法。
A group-specific, quantitative real-time PCR assay for detection of crab, a crustacean shellfish allergen, in complex food matrices.
机构信息
U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of Regulatory Science, 5100 Paint Branch Parkway, College Park, MD 20740, United States.
U.S. Food and Drug Administration, Center for Food Safety and Applied Nutrition, Office of Regulatory Science, 5100 Paint Branch Parkway, College Park, MD 20740, United States.
出版信息
Food Chem. 2018 Apr 1;244:224-231. doi: 10.1016/j.foodchem.2017.10.045. Epub 2017 Oct 13.
A real-time PCR assay was developed for detection of crab, a crustacean allergen, in food products. Group-specific primers and probes were developed to detect numerous species of crab. Method validation included tests of detection in complex food matrices, evaluation of commercial food products, and cross-reactivity testing on a wide variety of crustaceans. The method was able to detect several species of crab spiked into complex food matrices at levels ranging from 0.1 to 10 parts per million (weight/weight), worked equally well on different platforms, exhibited high specificity for crab over other types of crustaceans, and yielded much higher signals from commercial food products listing crab as an ingredient than from those containing other crustaceans.
建立了一种实时 PCR 检测方法，用于检测食品中的蟹过敏原。本研究设计了种属特异性引物和探针，以检测多种蟹类。方法验证包括在复杂食品基质中的检测测试、商业食品产品的评估和对各种甲壳类动物的交叉反应性测试。该方法能够检测到复杂食品基质中添加的几种蟹，水平范围为 0.1 至 10 百万分率（重量/重量），在不同平台上效果相同，对蟹具有很高的特异性，而对其他类型的甲壳类动物则没有特异性，并且从含有蟹作为成分的商业食品产品中获得的信号远高于那些含有其他甲壳类动物的产品。
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