Department of Grassland Science, South China Agricultural University, Guangzhou, China.
Anim Sci J. 2012 Feb;83(2):128-35. doi: 10.1111/j.1740-0929.2011.00937.x. Epub 2011 Aug 16.
Two lactic acid bacteria (LAB) strains, Pediococcus pentosaceus SC1 and Lactobacillus paraplantarum SC2 isolated from king grass silage, were characterized and their effectiveness to improve the silage fermentation quality of stylo (Stylosanthes guianensis Sw.) was studied. Strain SC1 was able to grow at a high temperature of 45°C, while SC2 did not. SC2 normally grew at a low pH of 4.0, while SC1 could not. These two strains and a commercial inoculant of LAB (L. plantarum, LP) were used as additives to stylo silage preparation at various temperatures (20°C, 30°C and 40°C). All LAB inoculants significantly (P < 0.05) reduced the pH value and ammonia-N content, and increased the ratio of lactic acid to acetic acid and quality score compared with the control. In addition, inoculating LAB strains markedly (P < 0.05) reduced butyric acid content at the temperatures of 30°C and 40°C. Compared to SC2 and LP strains, strain SC1 was the most effective for improving stylo silage quality at 20°C, indicated by the increase in lactic acid, ratio of lactic acid to acetic acid and quality score. At 30°C and 40°C, there were no significant differences among SC1, SC2 and LP treatments in pH values, contents of acetic acid, butyric acid and ammonia-N (P > 0.05).
从巨菌草青贮中分离得到的两株乳酸菌（LAB）菌株，戊糖片球菌 SC1 和植物乳杆菌 SC2，对其特性进行了研究，并研究了它们改善柱花草（Stylosanthes guianensis Sw.）青贮发酵品质的效果。菌株 SC1 能够在 45°C 的高温下生长，而 SC2 则不能。SC2 通常在低 pH 值 4.0 下生长，而 SC1 则不能。这两株菌和一种商业 LAB 接种剂（L. plantarum，LP）被用作不同温度（20°C、30°C 和 40°C）下柱花草青贮制备的添加剂。所有 LAB 接种剂均显著（P < 0.05）降低了 pH 值和氨态氮含量，并增加了乳酸与乙酸的比例和质量评分与对照组相比。此外，在 30°C 和 40°C 下接种 LAB 菌株显著（P < 0.05）降低了丁酸含量。与 SC2 和 LP 菌株相比，菌株 SC1 在 20°C 下对改善柱花草青贮质量最为有效，表现为乳酸、乳酸与乙酸的比例和质量评分增加。在 30°C 和 40°C 下，SC1、SC2 和 LP 处理之间的 pH 值、乙酸、丁酸和氨态氮含量均无显著差异（P > 0.05）。
