Li Mao, Zi Xuejuan, Zhou Hanlin, Lv Renlong, Tang Jun, Cai Yimin
Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences, Danzhou, 571737, Hainan, China.
College of Forestry, Hainan University, Danzhou, 571737, Hainan, China.
AMB Express. 2019 Nov 9;9(1):180. doi: 10.1186/s13568-019-0906-2.
To effectively utilize the tropical cassava (Manihot esculenta Crantz) foliage (CF) resources, the CF silages were prepared with microbial additives, including Chikuso-1 (CH1, Lactobacillus plantarum), Snow Lact L (SN, L. rhamnosus), Acremonium cellulase (CE), SN + CE and CH1 + CE. Silage fermentation, chemical composition and ruminal degradation were studied in Hainan, China. CF silages prepared with lactic acid bacteria (LAB) and CE were well preserved, with a higher (P < 0.05) lactic acid, a lower (P < 0.05) pH value, butyric acid content and NH3-N ⁄ total-N compared with the controls. The additive-treated silages showed increased crude protein (CP) content, but decreased (P < 0.05) NDF and ADF contents. Meanwhile, the additive treatment improved relative feed value and ruminal degradability of dry matter (DM), CP, neutral detergent fiber and acid detergent fiber. In addition, the combination of LAB and CE resulted in better fermentation quality and ruminal degradability compared with LAB or CE single treatment. The results demonstrated that the CF could be prepared as ruminant feed, and the combination of LAB and CE might exert beneficial synergistic effect on silage fermentation.
为有效利用热带木薯(Manihot esculenta Crantz)叶(CF)资源,用微生物添加剂制备了CF青贮料,包括Chikuso - 1(CH1,植物乳杆菌)、Snow Lact L(SN,鼠李糖乳杆菌)、顶头孢霉纤维素酶(CE)、SN + CE和CH1 + CE。在中国海南研究了青贮料发酵、化学成分和瘤胃降解情况。与对照相比,用乳酸菌(LAB)和CE制备的CF青贮料保存良好,乳酸含量较高(P < 0.05),pH值、丁酸含量和氨态氮/总氮较低(P < 0.05)。添加剂处理的青贮料粗蛋白(CP)含量增加,但中性洗涤纤维(NDF)和酸性洗涤纤维(ADF)含量降低(P < 0.05)。同时,添加剂处理提高了干物质(DM)、CP、中性洗涤纤维和酸性洗涤纤维的相对饲料价值和瘤胃降解率。此外,与LAB或CE单一处理相比,LAB和CE组合导致更好的发酵质量和瘤胃降解率。结果表明,CF可制备为反刍动物饲料,LAB和CE组合可能对青贮料发酵产生有益的协同作用。