Ramesh Veluchamy, Arivudainambi U, Thalavaipandian Annamalai, Karunakaran Chandran, Rajendran Ayyappan
Department of Botany, Biomedical Research Lab, VHNSN College, Virudhunagar - 626 001, India.
Int J Med Mushrooms. 2012;14(1):47-53. doi: 10.1615/intjmedmushr.v14.i1.50.
There is a growing need for new and effective antibiotic agents due to the recent emergence of life-threatening, multidrug-resistant bacterial infections such as methicillin-resistant Staphylococcus aureus and Pseudomonas aeruginosa. In the present study, the antimicrobial potential of mushroom was investigated against multidrug-resistant bacterial strains. The mushroom was identified as Xylaria sp. strain R005 based on the morphological characteristics and confirmed by 18S ribosomal RNA sequence comparisons. The crude ethyl acetate extracts of culture filtrate and fruiting bodies of Xylaria sp. showed significant antibacterial activity against multidrug-resistant S. aureus strains (1-10) and P. aeruginosa strains (1-8). The minimum inhibitory concentration of the ethyl acetate extracts of culture filtrate and fruiting bodies ranged from 225 µg/mL to 625 µg/mL, and 120 µg/mL to 625 µg/mL, respectively, against clinical strains of S. aurues and P. aeruginosa. The synergistic action of extracts of Xylaria sp. with vancomycin and ciprofloxacin was observed against S. aureus strain 6 and P. aeruginosa strain 3, respectively. The fractional inhibitory concentration indices (FICIs) of culture filtrate extract with vancomycin and ciprofloxacin were 0.5 and 0.18, respectively. The FICI of fruiting body extract with vancomycin and ciprofloxacin were 0.5 and 0.375, respectively. These results clearly indicate that the metabolites of culture filtrate and fruiting bodies of Xylaria sp. are the potential source for production of new antimicrobial compounds.
由于近期出现了如耐甲氧西林金黄色葡萄球菌和铜绿假单胞菌等危及生命的多重耐药细菌感染，对新型有效抗生素的需求日益增长。在本研究中，研究了蘑菇对多重耐药细菌菌株的抗菌潜力。根据形态特征将该蘑菇鉴定为炭角菌属菌株R005，并通过18S核糖体RNA序列比较进行了确认。炭角菌属的培养滤液和子实体的粗乙酸乙酯提取物对多重耐药金黄色葡萄球菌菌株（1 - 10）和铜绿假单胞菌菌株（1 - 8）显示出显著的抗菌活性。培养滤液和子实体的乙酸乙酯提取物对金黄色葡萄球菌和铜绿假单胞菌临床菌株的最低抑菌浓度分别为225 µg/mL至625 µg/mL和120 µg/mL至625 µg/mL。分别观察到炭角菌属提取物与万古霉素和环丙沙星对金黄色葡萄球菌菌株6和铜绿假单胞菌菌株3的协同作用。培养滤液提取物与万古霉素和环丙沙星的部分抑菌浓度指数（FICIs）分别为0.5和0.18。子实体提取物与万古霉素和环丙沙星的FICI分别为0.5和0.375。这些结果清楚地表明，炭角菌属培养滤液和子实体的代谢产物是生产新型抗菌化合物的潜在来源。
