Schillaci Domenico, Cusimano Maria Grazia, Cascioferro Stella Maria, Di Stefano Vita, Arizza Vincenzo, Chiaramonte Marco, Inguglia Luigi, Bawadekji Abdulhakim, Davino Salvatore, Gargano Maria Letizia, Venturella Giuseppe
Department STEBICEF, University of Palermo, Palermo, Italy.
Department STEBICEF, University of Palermo, Palermo, Italy; Euro Mediterranean Institute of Science and Technology (IEMEST), Palermo, Italy.
Int J Med Mushrooms. 2017;19(2):121-125. doi: 10.1615/IntJMedMushrooms.v19.i2.30.
Medicinal mushrooms represent an unlimited source of polysaccharides with nutritional, antitumoral, antibacterial, and immune-stimulating properties. Traditional studies of epigeous higher Basidiomycetes have recently been joined by studies of hypogeous fungi and, in particular, of so-called desert truffles. With the aim to obtain novel agents against bacteria of clinical importance, we focused on the edible desert truffle mushrooms Tirmania pinoyi, Terfezia claveryi, and Picoa juniperi as sources of new antimicrobial agents. In particular, we investigated the in vitro antibacterial activity of acid-soluble protein extracts (aqueous extracts) of these 3 species against the Gram-positive human pathogenic reference strain Staphylococcus aureus ATCC 29213 and the Gram-negative strain Pseudomonas aeruginosa ATCC 15442. The acid-soluble protein extracts of T. pinoyi and T. claveryi showed minimum inhibitory concentrations of 50 μg/mL against tested pathogens. We believe that such preliminary results are promising to obtain a valuable antibiotic alternative to fight antibiotic-resistant pathogens.
药用蘑菇是多糖的无限来源，具有营养、抗肿瘤、抗菌和免疫刺激特性。传统上对地上高等担子菌的研究，最近又有了对地下真菌，特别是所谓沙漠松露的研究。为了获得针对具有临床重要性细菌的新型药物，我们将重点放在可食用的沙漠松露蘑菇皮诺蒂尔马尼亚菌、克拉韦里地菇和刺柏皮库菌上，将它们作为新型抗菌剂的来源。特别是，我们研究了这3个物种的酸溶性蛋白提取物（水提取物）对革兰氏阳性人类致病参考菌株金黄色葡萄球菌ATCC 29213和革兰氏阴性菌株铜绿假单胞菌ATCC 15442的体外抗菌活性。皮诺蒂尔马尼亚菌和克拉韦里地菇的酸溶性蛋白提取物对受试病原体的最低抑菌浓度为50μg/mL。我们相信，这样的初步结果有望获得一种有价值的抗生素替代品，以对抗耐药病原体。
