Department of Biomedical Engineering, Columbia University, New York, New York, USA.
Biophys J. 2012 Jan 18;102(2):L5-7. doi: 10.1016/j.bpj.2011.12.011.
Mechanical forces play an increasingly recognized role in modulating cell function. This report demonstrates mechanosensing by T cells, using polyacrylamide gels presenting ligands to CD3 and CD28. Naive CD4 T cells exhibited stronger activation, as measured by attachment and secretion of IL-2, with increasing substrate elastic modulus over the range of 10-200 kPa. By presenting these ligands on different surfaces, this report further demonstrates that mechanosensing is more strongly associated with CD3 rather than CD28 signaling. Finally, phospho-specific staining for Zap70 and Src family kinase proteins suggests that sensing of substrate rigidity occurs at least in part by processes downstream of T-cell receptor activation. The ability of T cells to quantitatively respond to substrate rigidly provides an intriguing new model for mechanobiology.
机械力在调节细胞功能方面发挥着越来越重要的作用。本报告通过展示在不同的表面呈现配体的聚丙稀酰胺凝胶来证明 T 细胞的机械敏感性，这种方法可以测量 CD3 和 CD28 上的配体。在 10-200kPa 的范围内，随着基质弹性模量的增加，幼稚 CD4 T 细胞的附着和白介素-2 的分泌显示出更强的激活作用。通过在不同的表面呈现这些配体，本报告进一步表明，机械敏感性与 CD3 信号而非 CD28 信号更为密切相关。最后，磷酸化特异性标记 Zap70 和 Src 家族激酶蛋白表明，细胞受体激活下游的过程至少在一定程度上参与了对基质刚性的感知。T 细胞定量响应基质刚性的能力为机械生物学提供了一个有趣的新模型。
