Shan Ning-ning, Jiang Yu-jie, Sui Xiao-hui, Li Ying, Liu Xin, Xu Hong-zhi, Wang Xin
Department of Hematology, Provincial Hospital Affiliated to Shandong University, Jinan, China.
Zhonghua Xue Ye Xue Za Zhi. 2011 Nov;32(11):783-5.
To investigate the role of IL-18 and IL-18BP balance in aplastic anemia (AA).
A total of 29 AA patients and 22 controls were recruited in present research. The expressions of IL-18 and IL-18BP were measured by enzyme-linked immunosorbent assay (ELISA). The mRNA expressions of IL-18 and IL-18BP were measured in all subjects using real-time quantitative polymerase chain reaction (RT-PCR).
The levels of the IL-18 in plasma of AA and normals were (365.5 ± 160.6) pg/ml and (175.9 ± 92.8) pg/ml (P < 0.01); and the expression of IL-18 in severe AA patients (441.3 ± 116.9) pg/ml were higher than that in non-severe AA patients (326.4 ± 167.0) pg/ml (P < 0.05). The level of IL-18BP was increased in plasma of AA (1788.6 ± 523.8) pg/ml than in normals (1083.6 ± 489.6) pg/ml (P < 0.05). But the ratio of IL-18/IL-18BP in AA patients was much higher than that in controls (P < 0.05). RT-PCR revealed the levels of IL-18 and IL-18BP mRNA were up-regulated in AA patients when compared to controls, but the ratio of IL-18/IL-18BP was significantly elevated in AA patients.
IL-18/IL-18BP imbalance may play an important role in pathogenesis of AA and regulating the balance of IL-18 and IL-18BP may be a therapeutic approach to AA.
探讨白细胞介素-18（IL-18）与白细胞介素-18结合蛋白（IL-18BP）平衡在再生障碍性贫血（AA）中的作用。
本研究共纳入29例AA患者和22例对照。采用酶联免疫吸附测定（ELISA）法检测IL-18和IL-18BP的表达。运用实时定量聚合酶链反应（RT-PCR）检测所有受试者IL-18和IL-18BP的mRNA表达。
AA患者血浆中IL-18水平为（365.5±160.6）pg/ml，正常对照为（175.9±92.8）pg/ml（P<0.01）；重型AA患者IL-18表达水平为（441.3±116.9）pg/ml，高于非重型AA患者（326.4±167.0）pg/ml（P<0.05）。AA患者血浆中IL-18BP水平为（1788.6±523.8）pg/ml，高于正常对照（1083.6±489.6）pg/ml（P<0.05）。但AA患者IL-18/IL-18BP比值远高于对照（P<0.05）。RT-PCR显示，与对照相比，AA患者IL-18和IL-18BP的mRNA水平上调，但AA患者IL-18/IL-18BP比值显著升高。
IL-18/IL-18BP失衡可能在AA发病机制中起重要作用，调节IL-18与IL-18BP平衡可能是AA的一种治疗方法。
