Korean Zoonoses Research Institute, Chonbuk National University, Jeonju, Republic of Korea.
Zoonoses Public Health. 2012 Mar;59(2):77-82. doi: 10.1111/j.1863-2378.2011.01429.x. Epub 2011 Aug 21.
Twenty mammary lymph node samples were collected from cattle on a farm in the Republic of Korea. These cattle were serologically negative for Brucella by tube agglutination test (≤ 1:50) and serum agglutination test (≤ 1:50). Out of 20 lymph node samples, two samples were positive for Brucella growth on Brucella agar as well as blood agar. Tests for urease, hydrogen sulphide and reactions against monospecific sera A and M indicated that these two isolates (No. 15 and 16) belong to the genus Brucella. Genus specific, AMOS (abortus, melitensis, ovis, suis) and Bruce-ladder multiplex polymerase chain reaction (PCR) assays confirmed the Brucella isolates as either a B. abortus or a B. canis strain. This is the first report of the occurrence of a B. canis infection in cattle in Korea. More survey data are needed to determine whether B. canis is a significant aetiology in the cases of cattle brucellosis in Korea.
从韩国一个农场的 20 头奶牛中采集了 20 个乳腺淋巴结样本。这些奶牛的试管凝集试验（≤1:50）和血清凝集试验（≤1:50）均为布鲁氏菌血清学阴性。在 20 个淋巴结样本中，有两个样本在布鲁氏菌琼脂和血琼脂上呈布鲁氏菌生长阳性。脲酶、硫化氢和针对单特异性血清 A 和 M 的反应试验表明，这两种分离株（No.15 和 16）属于布鲁氏菌属。属特异性 AMOS（流产、马耳他、绵羊、猪）和 Bruce-ladder 多重聚合酶链反应（PCR）检测证实，这两种布鲁氏菌分离株为流产布鲁氏菌或犬布鲁氏菌株。这是首次在韩国报告牛发生犬布鲁氏菌感染。需要更多的调查数据来确定犬布鲁氏菌是否是韩国牛布鲁氏菌病病例的重要病因。
