适用于传统PCR和实时PCR系统的羊种布鲁氏菌、牛种布鲁氏菌、猪种布鲁氏菌、绵羊附睾种布鲁氏菌、犬种布鲁氏菌和沙鼠种布鲁氏菌的新型鉴定与区分
Novel identification and differentiation of Brucella melitensis, B. abortus, B. suis, B. ovis, B. canis, and B. neotomae suitable for both conventional and real-time PCR systems.
作者信息
Hinić V, Brodard I, Thomann A, Cvetnić Z, Makaya P V, Frey J, Abril C
机构信息
National Centre for Zoonoses, Bacterial Animal Diseases and Antimicrobial Resistance (ZOBA), Institute of Veterinary Bacteriology, University of Bern, Vetsuisse Faculty, Länggass-Strasse 122, P. O. Box, CH-3001 Bern, Switzerland.
出版信息
J Microbiol Methods. 2008 Oct;75(2):375-8. doi: 10.1016/j.mimet.2008.07.002. Epub 2008 Jul 15.
We describe the development of a novel PCR assay for the rapid detection of members of the Brucella genus, and the differentiation between six recognized Brucella species. The assay has proven to be highly specific with the additional advantage of being suitable for use with both conventional and real-time PCR.
我们描述了一种新型聚合酶链反应（PCR）检测方法的开发，该方法用于快速检测布鲁氏菌属成员，并区分六种已确认的布鲁氏菌物种。该检测方法已被证明具有高度特异性，并且还有一个额外的优点，即适用于传统PCR和实时PCR。
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