Department of Chemotherapy, Qilu Hospital, Shandong University School of Medicine, Jinan, Shandong 250012, China.
Chin Med J (Engl). 2011 Nov;124(21):3546-50.
Astrocyte elevated gene-1 (AEG-1), primarily identified as a late response gene induced by HIV-1 infection, plays multiple roles in the process of oncogenesis. This novel gene has been demonstrated to be involved in the several potent carcinogenic pathways, including PI3K/Akt pathway, nuclear factor (NF)-κB pathway, and Wnt/κ-catenin pathway. Although the function of AEG-1 has been intensively investigated in recent years, the molecular mechanism underlying its oncogenic role is largely unknown. The aim of this research was to explore the potential function of AEG-1 in breast cancer development and progression.
AEG-1 was ectopically overexpressed in breast cancer MCF-7 cells and its biological effects on the proliferation and invasion of MCF-7 cells were studied by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and invasion assays. The expression of HER2/neu, a crucial oncogene involving in breast cancer carcinogenesis, was also determined.
Overexpression of the AEG-1 promoted the proliferation and invasion ability of breast cancer cells, and upregulated the expression of HER2/neu, a crucial oncogene involving in breast cancer carcinogenesis.
AEG-1 might facilitate the proliferation and invasion of breast cancer cells by upregulating HER2/neu expression, which provides a potential target for breast cancer therapy.
星形细胞上调基因-1（AEG-1）最初被鉴定为 HIV-1 感染诱导的晚期反应基因，在肿瘤发生过程中发挥多种作用。该新基因已被证明参与了几种有效的致癌途径，包括 PI3K/Akt 途径、核因子（NF）-κB 途径和 Wnt/κ-catenin 途径。尽管近年来对 AEG-1 的功能进行了深入研究，但它致癌作用的分子机制在很大程度上仍不清楚。本研究旨在探讨 AEG-1 在乳腺癌发展和进展中的潜在功能。
通过 3-(4,5-二甲基噻唑-2-基)-2,5-二苯基四氮唑溴盐（MTT）和侵袭实验研究 AEG-1 在乳腺癌 MCF-7 细胞中过表达对 MCF-7 细胞增殖和侵袭的生物学影响。还测定了涉及乳腺癌发生的关键癌基因 HER2/neu 的表达。
AEG-1 的过表达促进了乳腺癌细胞的增殖和侵袭能力，并上调了涉及乳腺癌发生的关键癌基因 HER2/neu 的表达。
AEG-1 可能通过上调 HER2/neu 表达促进乳腺癌细胞的增殖和侵袭，为乳腺癌治疗提供了一个潜在的靶点。
