致瘤细胞在小鼠 MPNST 中很常见，但它们的频率取决于肿瘤基因型和检测条件。
Tumorigenic cells are common in mouse MPNSTs but their frequency depends upon tumor genotype and assay conditions.
机构信息
Department of Internal Medicine, Life Sciences Institute, and Center for Stem Cell Biology, University of Michigan, Ann Arbor, MI 48109-2216, USA.
出版信息
Cancer Cell. 2012 Feb 14;21(2):240-52. doi: 10.1016/j.ccr.2011.12.027.
Abstract
Tumor-initiating cells have been suggested to be rare in many cancers. We tested this in mouse malignant peripheral nerve sheath tumors (MPNSTs) and found that 18% of primary and 49% of passaged MPNST cells from Nf1(+/-); Ink4a/Arf(-/-) mice formed tumors upon transplantation, whereas only 1.8% to 2.6% of MPNST cells from Nf1(+/-); p53(+/-) mice did. MPNST cells of both genotypes require laminin binding to β1-integrin for clonogenic growth. Most MPNST cells from Nf1(+/-); Ink4a/Arf(-/-) mice expressed laminin, whereas most MPNST cells from Nf1(+/-); p53(+/-) mice did not. Exogenous laminin increased the percentage of MPNST cells from Nf1(+/-); p53(+/-) but not Nf1(+/-); Ink4a/Arf(-/-) mice that formed tumorigenic colonies. Tumor-forming potential is common among MPNST cells, but the assay conditions required to detect it vary with tumor genotype.
摘要
肿瘤起始细胞被认为在许多癌症中很少见。我们在鼠恶性外周神经鞘瘤（MPNST）中对此进行了测试，发现 Nf1(+/-); Ink4a/Arf(-/-) 小鼠的原发和传代 MPNST 细胞中有 18%形成肿瘤，而 Nf1(+/-); p53(+/-) 小鼠的 MPNST 细胞中仅有 1.8%至 2.6%形成肿瘤。两种基因型的 MPNST 细胞都需要层粘连蛋白与β1-整合素结合才能进行集落形成生长。Nf1(+/-); Ink4a/Arf(-/-) 小鼠来源的大多数 MPNST 细胞表达层粘连蛋白，而 Nf1(+/-); p53(+/-) 小鼠来源的大多数 MPNST 细胞不表达层粘连蛋白。外源性层粘连蛋白增加了 Nf1(+/-); p53(+/-) 但不增加 Nf1(+/-); Ink4a/Arf(-/-) 小鼠形成致瘤集落的 MPNST 细胞的比例。MPNST 细胞中存在肿瘤形成潜能，但检测它所需的实验条件因肿瘤基因型而异。
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