Institute of Medical and Biological Engineering, Faculty of Biological Sciences, The University of Leeds, Leeds LS2 9JT, UK.
Eur J Vasc Endovasc Surg. 2012 May;43(5):573-81. doi: 10.1016/j.ejvs.2012.01.027. Epub 2012 Feb 15.
Several studies have reported biological vascular grafts to be more resistant to microbial infection than synthetic counterparts in vivo. Indeed, small intestinal submucosa (SIS) materials have previously been reported to be antimicrobial. The aim of this study was to assess the antimicrobial activity and the ability to resist biofilm formation of a novel acellular vascular graft and compare it to commercially available alternatives using a range of organisms: MRSA, MSSA, Staphylococcus epidermidis, Enterococcus faecalis, Escherichia coli, Klebsiella pneumonia, Pseudomonas aeruginosa and Candida albicans. This was achieved using a modified disk diffusion assay and extraction of the materials into solution followed by minimum inhibitory concentration assays. To assess resistance to biofilm formation a novel biofilm assay was developed which compared the total colony forming units (CFU) recovered from each material and identification of the percentage of CFU which were loosely attached, residing within the biofilm or attached to the biomaterial. The results indicated a lack of antimicrobial activity for all materials tested, including SIS. The biological materials were more resistant to the formation of a biofilm compared to Dacron.
多项研究报告称，生物血管移植物在体内比合成移植物更能抵抗微生物感染。事实上，先前已有报道称，小肠黏膜下层（SIS）材料具有抗菌作用。本研究旨在评估一种新型去细胞血管移植物的抗菌活性和抗生物膜形成能力，并通过一系列生物体（MRSA、MSSA、表皮葡萄球菌、粪肠球菌、大肠杆菌、肺炎克雷伯菌、铜绿假单胞菌和白色念珠菌）将其与市售替代品进行比较。这是通过改良的圆盘扩散测定法和将材料提取到溶液中，然后进行最低抑菌浓度测定来实现的。为了评估抗生物膜形成的能力，开发了一种新型生物膜测定法，该方法比较了从每种材料中回收的总菌落形成单位（CFU），并确定了松散附着、存在于生物膜内或附着在生物材料上的 CFU 的百分比。结果表明，所有测试的材料（包括 SIS）均无抗菌活性。与 Dacron 相比，生物材料更能抵抗生物膜的形成。
