Perkinsus olseni（Perkinsozoa 门）中五个基因的染色体定位。
Chromosomal localisation of five genes in Perkinsus olseni (Phylum Perkinsozoa).
机构信息
Molecular Genetic Laboratory, Institute of Biomedical Sciences Abel Salazar, University of Porto, P-4099-003 Porto, Portugal.
出版信息
Eur J Protistol. 2012 Aug;48(3):194-8. doi: 10.1016/j.ejop.2011.11.002. Epub 2012 Feb 17.
The molecular karyotype of Perkinsus olseni, a pathogenic protist that infects the clam Ruditapes decussatus, comprises nine chromosomes, ranging in size from 0.15 Mb to 6.5 Mb, representing a haploid genome of about 28 Mb. In order to establish chromosome specific markers, PCR-amplified DNA sequences belonging to five conserved genes (18S rRNA, actin type I, hsp90, β-tubulin and calmodulin) were hybridised to chromosomal bands separated by pulsed-field gel electrophoresis. Three of those probes (actin type I, hsp90 and calmodulin) hybridised to only one chromosome and the remaining two (18S rRNA and β-tubulin) hybridised to two chromosomes. In the first place, the hybridisation pattern obtained serves to dispel any doubt about the nuclear location of the smallest chromosome observed in the molecular karyotype of Perkinsus olseni. Additionally, it will be a reference for further analysis of karyotype polymorphisms in the genus Perkinsus.
感染蛤类的致病性原生动物奥尔森派琴虫的分子核型由 9 条染色体组成，大小从 0.15Mb 到 6.5Mb 不等，代表大约 28Mb 的单倍体基因组。为了建立染色体特异性标记，我们对属于 5 个保守基因（18S rRNA、肌动蛋白 I 型、hsp90、β-微管蛋白和钙调蛋白）的 PCR 扩增 DNA 序列进行了杂交，这些序列与脉冲场凝胶电泳分离的染色体带杂交。其中 3 个探针（肌动蛋白 I 型、hsp90 和钙调蛋白）仅与 1 条染色体杂交，其余 2 个（18S rRNA 和 β-微管蛋白）与 2 条染色体杂交。首先，获得的杂交模式消除了奥尔森派琴虫分子核型中观察到的最小染色体的核定位的任何疑问。此外，它将成为进一步分析派琴虫属的核型多态性的参考。
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