Littoral Environnement et Sociétés, UMR 7266, CNRS-Université de La Rochelle, 2 rue Olympe de Gouges, F-17000 La Rochelle, France.
Chemosphere. 2012 May;87(8):938-44. doi: 10.1016/j.chemosphere.2012.01.049. Epub 2012 Feb 18.
The first objective of this study was to determine the bioaccumulation kinetics of pyrene in the soft tissues of Crassostrea gigas (mantle, muscle, gills, digestive gland, and the remaining soft tissues). As bivalves can biotransform hydrocarbons in more polar compounds (metabolites) that are more easily excreted, the second objective was to investigate the oyster capacity to metabolize pyrene into its metabolite, the 1-hydroxypyrene. To these ends, oysters were exposed 24 h to waterborne (14)C-pyrene then placed in depuration conditions for 15 d. Oysters efficiently bioaccumulated pyrene in their soft tissues and equilibrium was reached within the exposure time. The metabolite1-hydroxypyrene was also detected in oyster tissues but represented only 4-14% of the parent pyrene. At the end of the exposure period, the gills and the mantle showed the highest pyrene proportion of total soft tissue content, i.e. 47% and 26%, respectively. After 15 d of depuration, the mantle contained 32% and 30% of the remaining pyrene and 1-hydroxypyrene, respectively. As C. gigas did not display a high capacity for metabolizing pyrene, it can be considered as a good bioindicator species to survey and monitor pyrene contamination in the coastal marine environment.
本研究的首要目标是确定多环芳烃（PAHs）中芘在软体动物（贻贝）组织中的生物积累动力学（生物浓缩动力学）。由于双壳类动物能够将烃类转化为更易排泄的极性化合物（代谢物），因此，本研究的第二个目标是研究贻贝将芘代谢为 1-羟基芘的能力。为此，将贻贝暴露于水中的（14）C-芘 24 小时，然后将其置于净化条件下 15 天。贻贝能够有效地将芘生物浓缩在其软体组织中，在暴露时间内达到平衡。在贻贝组织中也检测到代谢物 1-羟基芘，但仅占母体芘的 4-14%。在暴露期结束时，鳃和贝壳显示出最高的总软体组织中芘的比例，分别为 47%和 26%。在 15 天的净化期后，贝壳分别含有 32%和 30%的剩余芘和 1-羟基芘。由于 C. gigas 对芘的代谢能力不高，因此可以将其视为一种良好的生物指示物种，用于调查和监测沿海海洋环境中的芘污染。
