Briner V A, Reinhart W H
Department of Internal Medicine, University of Berne, Inselspital Berne, Switzerland.
Nephron. 1990;56(1):13-8. doi: 10.1159/000186093.
We used an in vitro model consisting of a polycarbonate filter with pore diameters of 3 microns, through which red cells suspended in urine or buffer were pumped at a flow rate of 0.5 ml/min. pH and osmolality of the suspension were systematically changed within a range of pH 5-8 and 200-800 mosm/kg H2O. It was found that: (1) glomerular shapes could be produced by this filtration technique; (2) urine facilitated the production of glomerular shapes; (3) certain shapes occurred predominantly, at specific conditions of pH and osmolality and (4) hemolysis decreased with increasing osmolality or very low pH. These observations shed light on the mechanism by which glomerular red cell shapes are formed.
我们使用了一种体外模型，该模型由孔径为3微米的聚碳酸酯滤膜组成，将悬浮在尿液或缓冲液中的红细胞以0.5毫升/分钟的流速泵过该滤膜。悬浮液的pH值和渗透压在pH 5 - 8和200 - 800毫渗摩尔/千克H₂O范围内系统地变化。结果发现：（1）这种过滤技术可产生肾小球形状；（2）尿液有助于肾小球形状的产生；（3）在特定的pH值和渗透压条件下，某些形状占主导；（4）溶血随着渗透压的增加或极低的pH值而减少。这些观察结果揭示了肾小球红细胞形状形成的机制。
