焦磷酸测序在沙门氏菌快速鉴定中的应用。
Application of pyrosequencing for Salmonella enterica rapid identification.
机构信息
Microbiology Laboratory, Shijiazhuang Center for Disease Control and Prevention, 3 Likang Street, Shijiazhuang, Hebei, China.
出版信息
J Microbiol Methods. 2012 Apr;89(1):49-52. doi: 10.1016/j.mimet.2012.01.020. Epub 2012 Feb 9.
PMID:22342525
Abstract
Application of pyrosequencing of six Salmonella-specific genes as a rapid Salmonella identification method was tested. Primers for hns, hisJ and hilA had non-specific reactions with non-Salmonella strains. Primers for invA, iroB and fimY had specific PCR products and pyrosequences of Salmonella, suggesting that they were suitable for Salmonella rapid identification.
摘要
应用 6 个沙门氏菌特异性基因的焦磷酸测序作为一种快速沙门氏菌鉴定方法进行了测试。hns、hisJ 和 hilA 的引物与非沙门氏菌菌株有非特异性反应。invA、iroB 和 fimY 的引物具有沙门氏菌的特异性 PCR 产物和焦磷酸测序，表明它们适合沙门氏菌的快速鉴定。
Zotero 插件