Department of Experimental Zoology, Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Sławkowska 17, 31-016 Kraków, Poland.
Mol Phylogenet Evol. 2012 May;63(2):500-9. doi: 10.1016/j.ympev.2012.01.024. Epub 2012 Feb 8.
This is the first phylogenetic study of the intraspecific variability within Paramecium multimicronucleatum with the application of two-loci analysis (ITS1-5.8S-ITS2-5'LSU rDNA and COI mtDNA) carried out on numerous strains originated from different continents. The species has been shown to have a complex structure of several sibling species within taxonomic species. Our analysis revealed the existence of 10 haplotypes for the rDNA fragment and 15 haplotypes for the COI fragment in the studied material. The mean distance for all of the studied P. multimicronucleatum sequence pairs was p=0.025/0.082 (rDNA/COI). Despite the greater variation of the COI fragment, the COI-derived tree topology is similar to the tree topology constructed on the basis of the rDNA fragment. P. multimicronucleatum strains are divided into three main clades. The tree based on COI fragment analysis presents a greater resolution of the studied P. multimicronucleatum strains. Our results indicate that the strains of P. multimicronucleatum that appear in different clades on the trees could belong to different syngens.
这是首次应用两基因座分析（ITS1-5.8S-ITS2-5'LSU rDNA 和 COI mtDNA）对来自不同大洲的众多菌株进行的多乳泡草履虫种内变异性的系统发育研究。研究表明，该物种在分类种内具有复杂的几个姊妹种结构。我们的分析显示，在所研究的材料中 rDNA 片段存在 10 种单倍型，COI 片段存在 15 种单倍型。所有研究的多乳泡草履虫序列对的平均距离为 p=0.025/0.082（rDNA/COI）。尽管 COI 片段的变异较大，但 COI 衍生的系统发育树拓扑结构与基于 rDNA 片段构建的系统发育树拓扑结构相似。多乳泡草履虫菌株分为三个主要分支。基于 COI 片段分析的树对所研究的多乳泡草履虫菌株具有更高的分辨率。我们的结果表明，树上不同分支出现的多乳泡草履虫菌株可能属于不同的同宗。
