Tarcz Sebastian, Rautian Maria, Potekhin Alexey, Sawka Natalia, Beliavskaya Alexandra, Kiselev Andrey, Nekrasova Irina, Przyboś Ewa
Institute of Systematics and Evolution of Animals, Polish Academy of Sciences, Kraków 31-016, Sławkowska 17, Poland.
Laboratory of Protistology and Experimental Zoology, Faculty of Biology and Soil Science, St. Petersburg State University, Universitetskaya nab. 7/9, Saint Petersburg 199034, Russia.
Mol Phylogenet Evol. 2014 Apr;73:140-5. doi: 10.1016/j.ympev.2014.01.019. Epub 2014 Jan 28.
Paramecium putrinum (Claparede & Lachmann 1858) is one of the smallest (80-140 μm long) species of the genus Paramecium. Although it commonly occurs in freshwater reservoirs, no molecular studies of P. putrinum have been conducted to date. Herein we present an assessment of molecular variation in 27 strains collected from widely separated populations by using two selected DNA fragments (ITS1-5.8S-ITS2-5'LSU rDNA and COI mtDNA). Both the trees and haplotype networks reconstructed for both genome fragments show that the studied strains of P. putrinum form five main haplogroups. The mean distance between the studied strains is p-distance=0.007/0.068 (rDNA/COI) and exhibits similar variability as that between P. bursaria syngens. Based on these data, one could hypothesize that the clusters revealed in the present study may correspond to previously reported syngens and that there are at least five cryptic species within P. putrinum.
腐败草履虫（Claparede & Lachmann，1858年）是草履虫属中最小的物种之一（体长80 - 140微米）。尽管它常见于淡水水体中，但迄今为止尚未对腐败草履虫进行过分子研究。在此，我们通过使用两个选定的DNA片段（ITS1 - 5.8S - ITS2 - 5'LSU rDNA和COI mtDNA），对从广泛分布的种群中收集的27个菌株的分子变异进行了评估。针对这两个基因组片段构建的系统发育树和单倍型网络均显示，所研究的腐败草履虫菌株形成了五个主要的单倍型类群。所研究菌株之间的平均距离为p距离 = 0.007 / 0.068（rDNA / COI），其变异程度与绿草履虫宗之间的变异程度相似。基于这些数据，可以推测本研究中揭示的聚类可能对应于先前报道的宗，并且在腐败草履虫中至少存在五个隐存种。
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