Goodman J H, McGregor J M, Clendenon N R, Gahbauer R A, Barth R F, Soloway A H, Fairchild R G
Department of Surgery, Ohio State University, Columbus.
Neurosurgery. 1990 Sep;27(3):383-8. doi: 10.1097/00006123-199009000-00007.
This investigation attempts to determine whether increased survival time seen when the F98 glioma model is treated with boron neutron capture therapy (BNCT) is a result of inhibition of tumor growth caused by radiation-induced alterations in endothelial cells and normal tissue components. This indirect effect of radiation has been called the tumor bed effect. A series of tumor-bearing rats was studied, using a standardized investigational BNCT protocol consisting of 50 mg/kg of Na2B12H11SH injected intravenously 14 to 17 hours before neutron irradiation at 4 x 10(12) n/cm2. Ten rats, serving as controls, received no treatment either before or after tumor implantation. A second group of 10 rats was treated with BNCT 4 days before tumor implantation; these animals received no further treatment. The remaining group of 10 rats received no pretreatment but was treated with BNCT 10 days after implantation. Histological and ultrastructural analyses were performed in 2 animals from each group 17 days after implantation. Survival times of the untreated control animals (mean, 25.8 days) did not differ statistically from the survival times of the rats in the pretreated group (mean, 25.5 days). The rats treated with BNCT after implantation survived significantly longer (P less than 0.02; mean, 33.2 days) than the controls and the preirradiated animals. Tumor size indices calculated from measurements taken at the time of death were similar in all groups. These results indicate that, with this tumor model, BNCT does not cause a tumor bed effect in cerebral tissue. The therapeutic gains observed with BNCT result from direct effects on tumor cells or on the peritumoral neovascularity.
本研究旨在确定，当F98胶质瘤模型接受硼中子俘获疗法（BNCT）治疗时观察到的生存时间延长，是否是由于辐射引起的内皮细胞和正常组织成分改变导致肿瘤生长受到抑制的结果。辐射的这种间接效应被称为肿瘤床效应。使用标准化的研究性BNCT方案对一系列荷瘤大鼠进行了研究，该方案包括在中子辐照前14至17小时静脉注射50 mg/kg的Na2B12H11SH，中子辐照剂量为4×10(12) n/cm2。10只大鼠作为对照，在肿瘤植入前后均未接受治疗。第二组10只大鼠在肿瘤植入前4天接受BNCT治疗；这些动物不再接受进一步治疗。其余10只大鼠未进行预处理，但在植入后10天接受BNCT治疗。在植入后17天，对每组中的2只动物进行组织学和超微结构分析。未治疗的对照动物的生存时间（平均25.8天）与预处理组大鼠的生存时间（平均25.5天）在统计学上没有差异。植入后接受BNCT治疗的大鼠的存活时间明显长于对照组和预辐照动物（P小于0.02；平均33.2天）。根据死亡时的测量计算出的肿瘤大小指数在所有组中相似。这些结果表明，对于该肿瘤模型，BNCT不会在脑组织中引起肿瘤床效应。BNCT观察到的治疗效果来自对肿瘤细胞或肿瘤周围新生血管的直接作用。
