开发最大的海草——巨藻（Hydrocharitaceae）的微卫星引物。
Development of microsatellite primers of the largest seagrass, Enhalus acoroides (Hydrocharitaceae).
机构信息
Department of Environmental Sciences, East China Normal University, Shanghai 200062, China.
出版信息
Am J Bot. 2012 Mar;99(3):e99-e101. doi: 10.3732/ajb.1100412. Epub 2012 Feb 16.
PREMISE OF THE STUDY
Microsatellite primers were developed for the seagrass Enhalus acoroides to investigate genetic variation and identify clonal structure.
METHODS AND RESULTS
Four polymorphic loci and 32 monomorphic loci were developed in E. acoroides. Two to four alleles per locus were observed at the polymorphic loci across 60 individuals of two E. acoroides populations. The observed and expected heterozygosities within populations ranged from 0.100 to 0.5667 and from 0.0977 to 0.5079, respectively.
CONCLUSIONS
Our study revealed very low polymorphism in E. acoroides, even at the polymorphic loci. Nevertheless, these primers are a useful tool to study genetic variation, clonal structure, and mating system.
研究前提
为了研究遗传变异和鉴定克隆结构，我们为海草 Enhalus acoroides 开发了微卫星引物。
方法和结果
在 E. acoroides 中开发了四个多态性位点和 32 个单态性位点。在两个 E. acoroides 种群的 60 个个体中，每个多态性位点观察到 2 到 4 个等位基因。种群内的观察和预期杂合度范围分别为 0.100 到 0.5667 和 0.0977 到 0.5079。
结论
我们的研究表明，即使在多态性位点，E. acoroides 的多态性也非常低。然而，这些引物是研究遗传变异、克隆结构和交配系统的有用工具。
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