一套用于 （藤黄科） 海菖蒲的微卫星引物。
A set of microsatellite primers for Zostera japonica (Zosteraceae).
机构信息
Department of Environmental Sciences, East China Normal University, Shanghai 200062, China.
出版信息
Am J Bot. 2011 Sep;98(9):e236-8. doi: 10.3732/ajb.1100296. Epub 2011 Aug 24.
PREMISE OF THE STUDY
Polymorphic microsatellite primers were developed in the seagrass Zostera japonica to investigate genetic variation and to identify clonal structure. •
METHODS AND RESULTS
Thirteen polymorphic loci and 23 monomorphic loci were developed in Z. japonica. Two to 13 alleles per locus were observed at the polymorphic loci across 57 individuals of two Z. japonica populations. The observed and expected heterozygosities within populations ranged from 0.0000 to 1.0000 and from 0.0000 to 0.8542, respectively. •
CONCLUSIONS
Our study showed high-level polymorphism at the polymorphic loci in Z. japonica. These primers would be a powerful tool to study genetic variation, clonal structure, and mating systems.
研究前提
在海草鳗草中开发了多态性微卫星引物，以研究遗传变异并鉴定克隆结构。
方法与结果
鳗草中开发了 13 个多态性位点和 23 个单态性位点。在两个鳗草种群的 57 个个体中，观察到每个多态性位点有 2 到 13 个等位基因。种群内的观察和预期杂合度范围分别为 0.0000 到 1.0000 和 0.0000 到 0.8542。
结论
我们的研究表明鳗草中多态性位点具有高水平的多态性。这些引物将成为研究遗传变异、克隆结构和交配系统的有力工具。
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