Institute for Immunity, Transplantation and Infection, Stanford University School of Medicine, Stanford, California 94305, USA.
Nat Rev Immunol. 2012 Feb 17;12(3):191-200. doi: 10.1038/nri3158.
The heterogeneity in the healthy human immune system, and the immunological changes that portend various diseases, have been only partially described. Their comprehensive elucidation has been termed the 'Human Immunology Project'. The accurate measurement of variations in the human immune system requires precise and standardized assays to distinguish true biological changes from technical artefacts. Thus, to be successful, the Human Immunology Project will require standardized assays for immunophenotyping humans in health and disease. A major tool in this effort is flow cytometry, which remains highly variable with regard to sample handling, reagents, instrument setup and data analysis. In this Review, we outline the current state of standardization of flow cytometry assays and summarize the steps that are required to enable the Human Immunology Project.
健康人体免疫系统的异质性，以及预示各种疾病的免疫学变化，仅部分得到描述。对其进行全面阐释被称为“人类免疫学计划”。要准确测量人体免疫系统的变化，需要精确且标准化的检测方法来区分真正的生物学变化和技术伪影。因此，人类免疫学计划要想成功，就需要标准化的检测方法来对健康和疾病人群进行免疫表型分析。在这方面，流式细胞术是一项主要工具，但在样本处理、试剂、仪器设置和数据分析方面仍然存在很大差异。在这篇综述中，我们概述了流式细胞术检测方法的当前标准化状态，并总结了实现人类免疫学计划所需的步骤。
