Comparative Orthopaedic Research Laboratory, Département de Sciences Cliniques, Faculté de Médecine Vétérinaire, Université de Montréal, C.P. 5000, Saint-Hyacinthe (QC), J2S 7C6, Canada.
Osteoarthritis Cartilage. 2012 Jun;20(6):572-83. doi: 10.1016/j.joca.2012.02.004. Epub 2012 Feb 15.
To correlate degenerative changes in cartilage and subchondral bone in the third carpal bone (C3) of Standardbred racehorses with naturally occurring repetitive trauma-induced osteoarthritis.
Fifteen C3, collected from Standardbred horses postmortem, were assessed for cartilage lesions by visual inspection and divided into Control (CO), Early Osteoarthritis (EOA) and Advanced Osteoarthritis (AOA) groups. Two osteochondral cores were harvested from corresponding dorsal sites on each bone and scanned with a micro-computed tomography (CT) instrument. 2D images were assembled into 3D reconstructions that were used to quantify architectural parameters from selected regions of interest, including bone mineral density and bone volume fraction. 2D images, illustrating the most severe lesion per core, were scored for architectural appearance by blinded observers. Thin sections of paraffin-embedded decalcified cores stained with Safranin O-Fast Green, matched to the micro-CT images, were scored using a modified Mankin scoring system.
Subchondral bone pits with deep focal areas of porosity were seen more frequently in AOA than EOA but never in CO. Articular cartilage damage was seen in association with a reduction in bone mineral and loss of bone tissue. Histological analyses revealed significant numbers of microcracks in the calcified cartilage of EOA and AOA groups and a progressive increase in the score compared with CO bones.
The data reveal corresponding, progressive degenerative changes in articular cartilage and subchondral bone, including striking focal resorptive lesions, in the third carpal bone of racehorses subjected to repetitive, high impact trauma.
将种马第三腕骨（C3）软骨和软骨下骨的退行性变化与自然发生的重复性创伤诱导的骨关节炎相关联。
从死后的标准品种马中收集了 15 个 C3，通过肉眼观察评估软骨病变，并将其分为对照组（CO）、早期骨关节炎（EOA）和晚期骨关节炎（AOA）组。从每个骨的相应背侧部位采集两个骨软骨核，并使用微计算机断层扫描（CT）仪器进行扫描。将 2D 图像组装成 3D 重建，用于从选定的感兴趣区域量化结构参数，包括骨矿物质密度和骨体积分数。对每个核中最严重病变的 2D 图像进行盲法观察者评估的结构外观评分。与微 CT 图像匹配的石蜡包埋脱钙核的薄片用番红 O-快绿染色，使用改良的 Mankin 评分系统进行评分。
在 AOA 中比在 EOA 中更频繁地观察到带有深焦点多孔性的软骨下骨陷窝，但在 CO 中从未观察到。关节软骨损伤与骨矿物质减少和骨组织丧失有关。组织学分析显示，在 EOA 和 AOA 组的钙化软骨中存在大量微裂纹，与 CO 骨相比，评分显著增加。
数据显示，在经受重复性高冲击创伤的赛马的第三腕骨中，关节软骨和软骨下骨存在相应的进行性退行性变化，包括明显的局灶性吸收性病变。
