Smith J W, Torres J E, Holmquist N D
Am J Epidemiol. 1979 Aug;110(2):141-7. doi: 10.1093/oxfordjournals.aje.a112798.
Twenty-six women with invasive cervical cancer were examined for lymphocyte responsiveness to phytohemagglutinin (PHA) herpes simplex virus (HSV). Blood specimens were obtained from each patient before radiation therapy and separated into subpopulations by Ficoll-Hypaque centrifugation. Lymphocytes were cultured in RPMI-1640 containing autologous plasma and exposed to PHA, HSV-1, HSV-2, and control antigens. The results compared with those of women having negative Papanicolaou smears and matched to the cancer patients by age, race, and socioeconomic class revealed significant differences in blastogenic response (3H-thymidine) to HSV-2 antigens. Results from groups with known HSV-1 and HSV-2 infections indicated that differences were associated with the cancer patients having a higher frequency of HSV-2 infection. Results generally agreed with the findings of previous studies in which serologic test procedures were used.
对26名浸润性宫颈癌女性进行了检测，以观察其淋巴细胞对植物血凝素（PHA）和单纯疱疹病毒（HSV）的反应性。在放疗前从每位患者采集血液样本，并通过Ficoll-Hypaque离心法将其分离成亚群。淋巴细胞在含有自体血浆的RPMI-1640培养基中培养，并暴露于PHA、HSV-1、HSV-2和对照抗原。将结果与巴氏涂片阴性且在年龄、种族和社会经济阶层上与癌症患者匹配的女性的结果进行比较，发现对HSV-2抗原的增殖反应（3H-胸腺嘧啶核苷）存在显著差异。已知HSV-1和HSV-2感染组的结果表明，差异与癌症患者中HSV-2感染频率较高有关。结果总体上与先前使用血清学检测程序的研究结果一致。
