Meckler R L, Baron R, McLachlan E M
School of Physiology and Pharmacology, University of New South Wales, Kensington, Australia.
Neuroscience. 1990;36(3):823-9. doi: 10.1016/0306-4522(90)90025-y.
Immunohistochemical techniques have shown that the C-fragment of tetanus toxin injected into medial gastrocnemius muscle in the guinea-pig and rat, in addition to its retrograde transport in the axons of the somatomotor, sympathetic and sensory neurons supplying this muscle, is taken up and concentrated by terminal varicosities within pre- and paravertebral sympathetic ganglia at all thoracolumbar levels. Staining was absent in chronically denervated ganglia, demonstrating the specific association of the antigen with preganglionic varicosities. Preganglionic varicosities at all levels were also labelled after C-fragment injection into the peritoneal cavity or into denervated medial gastrocnemius; both of these procedures failed to label somatomotor or sensory neurons. Although retrograde trans-synaptic transport could be demonstrated, sympathetic labelling was sparse and non-specific, so that the C-fragment of tetanus toxin is unsuitable for the identification of sympathetic pathways. The selective and widespread uptake of tetanus toxin by sympathetic preganglionic terminals could explain the diverse autonomic symptoms observed in tetanus intoxication.
免疫组织化学技术显示，将破伤风毒素的C片段注射到豚鼠和大鼠的腓肠肌内侧后，除了在供应该肌肉的躯体运动、交感和感觉神经元的轴突中逆行运输外，它还被胸腰段所有水平的椎前和椎旁交感神经节内的终末膨体摄取并浓缩。在慢性去神经节中没有染色，这表明抗原与节前膨体有特异性关联。将C片段注射到腹腔或去神经的腓肠肌内侧后，所有水平的节前膨体也被标记；这两种方法都未能标记躯体运动或感觉神经元。虽然可以证明逆行跨突触运输，但交感神经标记稀疏且非特异性，因此破伤风毒素的C片段不适合用于识别交感神经通路。交感神经节前终末对破伤风毒素的选择性和广泛摄取可以解释破伤风中毒时观察到的各种自主神经症状。
