Schwab M, Thoenen H
Brain Res. 1977 Feb 25;122(3):459-74. doi: 10.1016/0006-8993(77)90457-7.
Adult rats were injected unilaterally into the anterior eye chamber and the submandibular gland with either [125I]tetanus toxin or [125I]nerve growth factor (NGF). Fourteen and 24 h later in electron microscopic autoradiographs of the superior cervical ganglia of the injected side the labeling was confined to a limited number (15-20%) of adrenergic ganglion cells and the silver grains were localized over axons, perikarya and dendrites providing evidence for a retrograde intraaxonal transport of the two macromolecules. Moreover, after injection of [125]tetanus toxin there was a very marked labeling of the presynaptic cholinergic nerve terminals. In contrast, after [125I]NGF these terminals were free of label. In both cases no specific labeling could be detected over glia and extracellular space. In the postganglionic axons the radioactivity seemed to be mainly associated with vesicles and smooth endoplasmic reticulum, in the perikarya and dendrites of the adrenergic neurons with secondary lysosomes, vesicles and smooth endoplasmic reticulum. The Golgi cisternae and the nuclei were free of radioactivity. The specific labeling of presynaptic terminals after injection of [125I]tetanus toxin together wirans-symaptic migration of [125I]tetanus toxin from the adrenergic ganglion cell to its innervating presynaptic terminals following retrograde intraaxonal transport.
将成年大鼠的前房和下颌下腺单侧注射[125I]破伤风毒素或[125I]神经生长因子（NGF）。在注射后14小时和24小时，在注射侧颈上神经节的电子显微镜放射自显影片中，标记仅限于有限数量（15 - 20%）的肾上腺素能神经节细胞，并且银颗粒定位于轴突、胞体和树突上，这为这两种大分子的逆行轴突内运输提供了证据。此外，注射[125I]破伤风毒素后，突触前胆碱能神经末梢有非常明显的标记。相比之下，注射[125I]NGF后，这些末梢没有标记。在这两种情况下，在神经胶质和细胞外空间均未检测到特异性标记。在节后轴突中，放射性似乎主要与囊泡和平滑内质网相关，在肾上腺素能神经元的胞体和树突中，放射性与次级溶酶体、囊泡和平滑内质网相关。高尔基体池和细胞核没有放射性。注射[125I]破伤风毒素后突触前末梢的特异性标记以及[125I]破伤风毒素在逆行轴突内运输后从肾上腺素能神经节细胞向其支配的突触前末梢的跨突触迁移。
