Dong Jin-Run, Guo Li-Yun, Qu Jia-Gui, Qi Ren-Li, Wang Wen-Chao, Xiao Chun-Jie, Wang Zheng-Bo
Institute of Biophysics, the Chinese Academy of Sciences, Beijing, China.
Dongwuxue Yanjiu. 2012 Feb;33(1):43-8. doi: 10.3724/SP.J.1141.2012.01043.
To investigate the characteristics of rhesus monkey embryonic stem cells and to promote their clinical application, the differentiation and proliferation of rosettes neural stem cells from GFP marked rhesus monkey embryonic stem cells were studied The results showed that: 1) A stable and high-efficient neural differentiation system was established. More than 95% of the embryonic stem cells were differentiated into neural stem cells on the 12(th) days of differentiation; 2) the rosettes neural stem cells differentiated from the rhesus monkey embryonic stem cells could maintain their rosettes-shape by proliferating with bFGF/EGF; 3) the neural stem cells could differentiate into neurons after transplanted into the rhesus monkey brain. In conclusion, the rosettes neural stem cells differentiated from rhesus monkey embryonic stem cells could maintain their characteristics after proliferation with bFGF/EGF and they could survive and differentiate into neurons after transplanted into the rhesus monkey brain, which strongly supports the clinical application of neural stem cells in the future.
为研究恒河猴胚胎干细胞的特性并促进其临床应用，对绿色荧光蛋白标记的恒河猴胚胎干细胞来源的玫瑰花结神经干细胞的分化和增殖进行了研究。结果显示：1）建立了稳定高效的神经分化体系。在分化第12天时，超过95%的胚胎干细胞分化为神经干细胞；2）恒河猴胚胎干细胞分化而来的玫瑰花结神经干细胞在添加碱性成纤维细胞生长因子/表皮生长因子的条件下增殖时可维持其玫瑰花结形态；3）将神经干细胞移植到恒河猴脑内后可分化为神经元。总之，恒河猴胚胎干细胞分化而来的玫瑰花结神经干细胞在添加碱性成纤维细胞生长因子/表皮生长因子的条件下增殖后可维持其特性，移植到恒河猴脑内后能够存活并分化为神经元，这为未来神经干细胞的临床应用提供了有力支持。
