细胞外肿瘤坏死因子会导致黑尾海兔一种特定神经元的钾离子电导降低。

Extracellular tumor necrosis factor induces a decreased K+ conductance in an identified neuron of Aplysia kurodai.

作者信息

Sawada M, Hara N, Maeno T

机构信息

Department of Physiology, Shimane Medical University, Izumo, Japan.

出版信息

Neurosci Lett. 1990 Jul 31;115(2-3):219-25. doi: 10.1016/0304-3940(90)90458-l.

DOI:10.1016/0304-3940(90)90458-l

PMID:2234501

Abstract

Recombinant human tumor necrosis factor (rhTNF) was pressure-applied onto the the soma of identified neuron R12 in the Aplysia abdominal ganglion. rhTNF induced a slow inward current (ITNF, 80-100 s in duration, 5-10 nA in amplitude) associated with a conductance decrease. ITNF begins 1-2 s after applying rhTNF and peaks in 5-6 s. ITNF was decreased by hyperpolarization and had a reversal potential of approximately -87 mV (close to the K+ equilibrium potential). Ion substitution and pharmacological experiments suggest that ITNF is due to a decreased K+ conductance and that TNF, a product of macrophages, may form an important link in communications between nervous and immune systems.

摘要

将重组人肿瘤坏死因子（rhTNF）施加于海兔腹神经节中已识别的神经元R12的胞体上。rhTNF诱导出一种缓慢内向电流（ITNF，持续时间为80 - 100秒，幅度为5 - 10纳安），伴随着电导降低。ITNF在施加rhTNF后1 - 2秒开始，5 - 6秒达到峰值。ITNF因超极化而降低，其反转电位约为 - 87毫伏（接近钾离子平衡电位）。离子置换和药理学实验表明，ITNF是由于钾离子电导降低所致，并且巨噬细胞产生的肿瘤坏死因子（TNF）可能在神经和免疫系统之间的通讯中形成重要联系。