Desmond E P, Schmidt N J, Lennette E H
Am J Trop Med Hyg. 1979 Jul;28(4):729-32.
Indirect immunoperoxidase (IP) staining was evaluated for sensitivity and specificity in detecting Colorado tick fever (CTF) virus antigen in infected cell cultures and infected mouse tissues, and then was applied to a study of congenital CTF infection in mice. The sensitivity of IP staining was comparable to that of immunofluorescence staining in detecting CTF antigen in infected cell cultures. Endogenous peroxidase activity of mouse tissues caused nonspecific reactivity in the IP system, but this could be abolished by treatment with sodium azide and hydrogen peroxide without destroying CTF antigen. Offspring of mice infected with CTF virus during the 2nd week of pregnancy showed a highly significant increase in the incidence of stillbirths and neonatal deaths as compared with offspring of uninfected controls. CTF antigen or virus was demonstrable in only a low proportion (7%) of embryos, ill newborns or stillborns examined, but a high proportion of mice examined at a time when maternal antibody would be lost (6 and 12 weeks) showed CTF antibody, indicating a higher incidence of infection. IP staining showed potential for use in studies of viral pathogenesis in the mouse model.
评估了间接免疫过氧化物酶（IP）染色在检测感染细胞培养物和感染小鼠组织中科罗拉多蜱传热（CTF）病毒抗原时的敏感性和特异性，然后将其应用于小鼠先天性CTF感染的研究。在检测感染细胞培养物中的CTF抗原时，IP染色的敏感性与免疫荧光染色相当。小鼠组织的内源性过氧化物酶活性在IP系统中引起非特异性反应，但通过用叠氮化钠和过氧化氢处理可以消除这种反应，而不会破坏CTF抗原。与未感染对照组的后代相比，在怀孕第2周感染CTF病毒的小鼠的后代死产和新生儿死亡的发生率显著增加。在检查的胚胎、患病新生儿或死产中，只有一小部分（7%）可检测到CTF抗原或病毒，但在母体抗体消失时（6周和12周）检查的高比例小鼠显示有CTF抗体，表明感染发生率更高。IP染色显示出在小鼠模型中病毒发病机制研究中的应用潜力。
