Carabin H, Gyorkos T W, Kokoskin E, Payment P, Joseph L, Soto J
Department of Epidemiology and Biostatistics, McGill University, Montreal, Quebec.
Can J Infect Dis. 1998 May;9(3):149-56. doi: 10.1155/1998/613048.
To compare three sampling methods and to pretest methods for the determination of fecal coliform (FC) counts and Toxocara species from sand in the day care outdoor environment.
The sand samples were obtained from the play area and the sandbox of a day care centre and examined for the presence of FC and Toxocara species, the common roundworm of dogs and cats. The sampling methods included random selection and two types of judgement methods. The latter included one method where domestic animals were judged to be likely to defecate and the other where children would be likely to be playing. In addition, to obtain a global estimate of contamination, the entire areas of both the sandbox and the play area were sampled on the last day.
Outdoor day care environment.
The most representative levels of bacterial contamination and Toxocara species originated from the combined sample of the entire surface areas rather than from any separate random or judgement method of sampling. FCs were found in all sampled areas of the sandbox (median 910 FCs/g of sand) and of the play area (median 350 FCs/g of sand). Toxocara species were recovered from a number of areas in both the sandbox and the play area.
Research on environmental microbial contamination of outdoor day care settings would benefit from the application of standardized and validated sampling and laboratory methods.
比较三种采样方法，并对日间照料室外环境中沙子里粪大肠菌群（FC）计数及弓首蛔虫属物种的检测方法进行预试验。
从一家日间照料中心的游乐区和沙箱采集沙子样本，检测其中FC及弓首蛔虫属物种（犬猫常见的蛔虫）的存在情况。采样方法包括随机选择以及两种判断方法。后者包括一种判断家畜可能排便地点的方法和另一种判断儿童可能玩耍地点的方法。此外，为了获得污染情况的总体估计，在最后一天对沙箱和游乐区的整个区域进行了采样。
室外日间照料环境。
细菌污染和弓首蛔虫属物种的最具代表性水平源自整个表面区域的混合样本，而非任何单独的随机或判断性采样方法。在沙箱的所有采样区域（中位数为每克沙子910个FC）和游乐区的所有采样区域（中位数为每克沙子350个FC）均发现了FC。在沙箱和游乐区的多个区域均检测到弓首蛔虫属物种。
对室外日间照料场所环境微生物污染的研究将受益于标准化和经过验证的采样及实验室方法的应用。
