Waindok Patrick, Raulf Marie-Kristin, Strube Christina
Institute for Parasitology, Centre for Infection Medicine, University of Veterinary Medicine Hannover, Hanover, Germany.
Food Waterborne Parasitol. 2022 Aug 9;28:e00174. doi: 10.1016/j.fawpar.2022.e00174. eCollection 2022 Sep.
Ascarid infections constitute a major concern for both human and animal health risk assessment. Although being effectively transmitted by soil, water and contaminated food, reliable detection of ascarid eggs in environmental media often remains challenging. However, contamination of the environment with ascarid ova has gained more attention as a decisive part of proper risk assessment in recent years. Due to various factors, such as sample matrices, dissociation detergents and flotation solutions, defined and standardised protocols for the isolation of eggs from complex environmental matrices are difficult to establish and therefore limited. Thus, this study reviews common techniques used for the recovery of ascarid eggs from environmental media with special emphasis on sampling strategies, purification procedures and microscopic as well as molecular detection of egg contamination. Despite various advancements, mainly in the field of molecular methods leading to more reliable and sensitive detection, it can be concluded that there is still a need for unified guidelines for sampling and recovery of ascarid eggs derived from complex environmental matrices.
蛔虫感染是人类和动物健康风险评估的主要关注点。尽管蛔虫可通过土壤、水和受污染的食物有效传播，但在环境介质中可靠检测蛔虫卵往往仍具有挑战性。然而，近年来，蛔虫卵对环境的污染作为适当风险评估的决定性部分受到了更多关注。由于各种因素，如样品基质、解离洗涤剂和浮选溶液，很难建立从复杂环境基质中分离蛔虫卵的明确和标准化方案，因此这类方案有限。因此，本研究回顾了从环境介质中回收蛔虫卵的常用技术，特别强调了采样策略、纯化程序以及蛔虫卵污染的显微镜检测和分子检测。尽管取得了各种进展，主要是在分子方法领域，从而实现了更可靠和灵敏的检测，但可以得出结论，对于从复杂环境基质中采样和回收蛔虫卵，仍然需要统一的指导方针。
