Farahnak A, Golmohamadi T, Rad Mb Molaei
Department of Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Parasitol. 2010 Mar;5(1):20-4.
Fascioliasis is a chronic hepatic disease and may be resulted from mechanical/molecular parasite adhesion to host liver tissue. The aim of this study was to detect surface carbohydrate and lectin, carbohydrate-binding protein isolation that might be responsible of this molecular binding.
The present experimental work was conducted in the Department of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran. Fasciola hepatica parasites were collected from abattoir (Saman, Tehran, Iran) and surface mannose-carbohydrate was detected by fluorescein isothiocyanate (FITC) conjugated lectin (Lentil). Lectin of tegumental tissue from F. hepatica was isolated by affinity chromatography and detected by sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE).
Mannose carbohydrate was observed on the surface of tegumental tissue from parasite under fluorescence microscope. Carbohydrate-binding protein or lectin with MW of 50 kDa also was isolated from homogenized tegument of helminth.
These results are important for understanding of molecular pathogenesis of F. hepatica at the chronic phase of fascioliasis.
肝片吸虫病是一种慢性肝脏疾病，可能由寄生虫与宿主肝组织的机械性/分子性黏附引起。本研究的目的是检测可能与这种分子结合有关的表面碳水化合物和凝集素，即碳水化合物结合蛋白。
本实验研究在伊朗德黑兰医科大学公共卫生学院医学寄生虫学与真菌学系进行。从屠宰场（伊朗德黑兰的萨曼）收集肝片吸虫寄生虫，并用异硫氰酸荧光素（FITC）偶联凝集素（扁豆凝集素）检测表面甘露糖碳水化合物。通过亲和层析分离肝片吸虫皮层组织的凝集素，并通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）进行检测。
在荧光显微镜下观察到寄生虫皮层组织表面存在甘露糖碳水化合物。还从蠕虫匀浆的皮层中分离出分子量为50 kDa的碳水化合物结合蛋白或凝集素。
这些结果对于理解肝片吸虫病慢性期肝片吸虫的分子发病机制具有重要意义。
