Heidari Z, Mohebali M, Zarei Z, Aryayipour M, Eshraghian Mr, Kia Eb, Shodajei S, Abdi J, Rakhshanpour A, Rokni Mb
Dept. of Medical Parasitology and Mycology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran.
Iran J Parasitol. 2011 Aug;6(3):19-25.
The aim of this study was to conduct a sero-epidemiological survey in Meshkinshahr, Ardabil Province, northwestern Iran to detect the rate of hydatidosis in the city and nearby villages. Literature shows that no such study has been conducted so far.
Overall, 670 serum samples were collected from 194 males and 476 females from patients referred to different health centers of the region. All patients filled out a questionnaire and an informed consent. Sera were analyzed using indirect-ELISA test. Ten µg /ml antigens (Antigen B derived from hydatid cyst fluid), serum dilutions of 1:500 and conjugate anti-human coombs with 1:10000 dilutions were utilized to perform the test. Data analysis was conducted using SPSS software ver. 11.5.
The seroprevalence of human hydatidosis was 1.79% by ELISA test in the region. This rate for females was 1.68% and males 2.6%, respectively. There was no significant difference as regards all factors studied and the seropositivity. According to job, farmers and ranchmen had the highest rate of infection as 3.17%. The sero-prevalence of infection was 2.6%% in illiterate people which showed the highest rate. As regards residency, urban life showed no significant difference with rural life (1.1% vs. 2.58%). Age group of 69-90 yr old, with 4.62% as prevalence had the highest rate of positivity.
Obtained sero-prevalence of hydatidosis shows more or less a resemblance to other cities of Iran, although due to the specific condition of the city we expected more rate of sero-positivity.
本研究旨在对伊朗西北部阿尔达比勒省梅什金沙赫尔市进行血清流行病学调查，以检测该市及周边村庄的包虫病感染率。文献表明，迄今为止尚未进行过此类研究。
总共从该地区不同健康中心转诊的患者中收集了670份血清样本，其中男性194例，女性476例。所有患者均填写了问卷并签署了知情同意书。血清采用间接酶联免疫吸附测定（ELISA）试验进行分析。使用10μg/ml抗原（源自包虫囊肿液的抗原B）、1:500的血清稀释度和1:10000稀释度的抗人库姆斯结合物进行试验。使用SPSS软件11.5版进行数据分析。
通过ELISA试验，该地区人类包虫病的血清阳性率为1.79%。女性的这一比率为1.68%，男性为2.6%。在所研究的所有因素与血清阳性之间没有显著差异。按职业划分，农民和牧民的感染率最高，为3.17%。文盲人群的感染血清阳性率为2.6%，为最高。就居住情况而言，城市生活与农村生活没有显著差异（1.1%对2.58%）。69 - 90岁年龄组的阳性率最高，为4.62%。
获得的包虫病血清阳性率与伊朗其他城市大致相似，尽管由于该市的特殊情况，我们预期血清阳性率会更高。
