RANTES 基因-403G/A 和-28C/G 多态性与黎巴嫩哮喘的相关性研究。

Study of the association between -403G/A and -28C/G RANTES gene polymorphisms and asthma in Lebanon.

机构信息

Department of Pharmacology, Faculty of Pharmacy, Beirut Arab University, Riyadh, Saudi Arabia.

出版信息

Ann Thorac Med. 2012 Jan;7(1):16-20. doi: 10.4103/1817-1737.91558.

DOI:10.4103/1817-1737.91558

PMID:22347345

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3277035/

Abstract

CONTEXT

Asthma is a complex inflammatory condition often associated with bronchial hyper reactivity and atopy. Genetic and environmental factors are implicated in the etiopathogenesis of asthma. Regulated upon Activation Normal T- cell Expressed and Secreted (RANTES) is a CC chemokine responsible for the recruitment of inflammatory cells, suggesting a possible role for this chemokine in asthma. Both -403A and -28G alleles of the RANTES promoter region were found to be associated with asthma/atopy in some but not all studies.

AIM

The purpose of this study was to investigate the genetic influence of -403A and -28G alleles of the RANTES promoter region on the development of asthma in Lebanon.

SETTINGS AND DESIGN

This case control study was conducted at Makassed Hospital, Beirut on 40 asthmatic patients and 38 healthy controls.

METHODS

RANTES gene polymorphisms -403G/A and -28C/G alleles were genotyped using PCR-RFLP.

RESULTS

No significant differences in allele or genotype frequencies for the RANTES gene polymorphisms between asthmatic patients and controls were found. The difference of the -403 GA genotype frequency between patients and controls was not statisti-cally significant; (OR=0.8, 95% CI=0.2-2.3, P=0.8). Similarly, the difference of the A-allele frequencies between patients and con-trols was not significant (OR=0.824, CI=0.3-2.2, P=0.7).

CONCLUSIONS

Our data show that RANTES gene promoter polymorphisms are not associated with asthma susceptibility in the Lebanese population.

摘要

背景

哮喘是一种复杂的炎症性疾病，常伴有支气管高反应性和过敏。遗传和环境因素都与哮喘的发病机制有关。调节激活正常 T 细胞表达和分泌（RANTES）是一种 CC 趋化因子，负责招募炎症细胞，提示这种趋化因子在哮喘中可能发挥作用。RANTES 启动子区域的-403A 和-28G 等位基因都与一些但不是所有研究中的哮喘/过敏有关。

目的

本研究旨在探讨 RANTES 启动子区域的-403A 和-28G 等位基因对黎巴嫩哮喘发病的遗传影响。

设置和设计

这项病例对照研究在贝鲁特 Makassed 医院进行，共纳入 40 名哮喘患者和 38 名健康对照者。

方法

采用 PCR-RFLP 技术检测 RANTES 基因多态性-403G/A 和-28C/G 等位基因。

结果

哮喘患者和对照组之间 RANTES 基因多态性的等位基因或基因型频率无显著差异。患者和对照组之间-403GA 基因型频率的差异无统计学意义（OR=0.8，95%CI=0.2-2.3，P=0.8）。同样，患者和对照组之间 A 等位基因频率的差异也无统计学意义（OR=0.824，CI=0.3-2.2，P=0.7）。