Goudarzi H, Mirsamadi Es, Farnia P, Jahani Sherafat S, Esfahani M, Faramarzi N
Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Iran J Microbiol. 2010 Dec;2(4):194-7.
Phospholipase of Mycobacterium tuberculosis plays an important role in pathogenesis through breaking up phospholipids and production of diacylglycerol. In this study, we examined the Beijing strains of Mycobacterium tuberculosis isolated from Iranian patients for the genes encoding this enzyme.
DNA extraction was performed using CTAB (cetyltrimethylammonium bromide) from positive culture specimens in tuberculosis patients. PCR was then used to amplify the plcA, plcB, plcC genes of Beijing strain, and non-Beijing strains were identified by spoligotyping.
Of 200 specimens, 19 (9.5%) were Beijing strain and 181 (90.5%) were non-Beijing strains. The results of PCR for Beijing strains were as follows: 16 strains (84.2%) were positive for plcA, 17 (89.4%) were positive for plcB and 17 (89.4%) were positive for plcC genes. The standard strain (H37RV) was used as control.
The majority of Beijing strains have phospholipase C genes which can contribute to their pathogenesis but we need complementary studies to confirm the role of phospholipase C in pathogenecity of Mycobacterium tuberculosis.
结核分枝杆菌的磷脂酶通过分解磷脂和产生二酰甘油在发病机制中发挥重要作用。在本研究中，我们检测了从伊朗患者中分离出的结核分枝杆菌北京菌株中编码该酶的基因。
使用十六烷基三甲基溴化铵（CTAB）从结核病患者的阳性培养标本中提取DNA。然后用聚合酶链反应（PCR）扩增北京菌株的plcA、plcB、plcC基因，并通过间隔寡核苷酸分型鉴定非北京菌株。
在200份标本中，19份（9.5%）为北京菌株，181份（90.5%）为非北京菌株。北京菌株的PCR结果如下：16株（84.2%）plcA基因阳性，17株（89.4%）plcB基因阳性，17株（89.4%）plcC基因阳性。以标准菌株（H37RV）作为对照。
大多数北京菌株具有磷脂酶C基因，这可能有助于其发病机制，但我们需要补充研究来证实磷脂酶C在结核分枝杆菌致病性中的作用。
