Borji Soroush, Kooti Sara, Ramazanzadeh Rashid, Kadivarian Sepide, Atashi Sara, Mohajeri Parviz
Student Research Committee, Department of Bacteriology and Virology, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran.
Environment Technologies Research Center, Medical Basic Sciences Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran.
J Clin Tuberc Other Mycobact Dis. 2024 Jul 26;36:100467. doi: 10.1016/j.jctube.2024.100467. eCollection 2024 Aug.
Tuberculosis (TB) is a chronic infectious disease with multiple manifestations and gradual progression that remains a major health problem and a leading cause of death worldwide. In recent years, a number of DNA fingerprinting techniques have been developed to identify strains of the (MTB) complex. Spoligotyping is one of the first PCR-based genotyping methods. Information on the number and identification of common strains among MTB complex samples in clinical samples from Kermanshah city is needed to develop more effective therapeutic strategies. This is a descriptive cross-sectional study of 41 sample patients with TB referred to Kermanshah Tuberculosis Centre between December 2021 and June 2022, including sputum, aspiration, urine, etc. First, the susceptibility of the developed bacteria to culture media was compared with that of isoniazid using the proportional method, and rifampin was determined according to the standard protocol. Demographic data of patients referred to the Centre for the Control of Lung Diseases were also recorded. In the next step, spoligotyping was carried out using the standard method and each strain pattern was recorded as an OCTAL code and compared with the information available at the World Bank on spoligotyping and its strains. Forty-one patients with pulmonary TB were tested using spoligotyping. Four MTB strains were identified, including H4, CAS, T1 and H1. The H4 strain also had the highest frequency with 16 samples (39%) among the MTB complex strains isolated using spoligotyping. The highest frequency of strains isolated using spoligotyping was associated with the H4 strain. It can be concluded that spoligotyping is very cost effective, simple, repeatable and highly sensitive.
结核病（TB）是一种具有多种表现形式且逐渐进展的慢性传染病，它仍然是一个主要的健康问题，也是全球主要的死亡原因之一。近年来，已开发出多种DNA指纹技术来鉴定结核分枝杆菌（MTB）复合群的菌株。寡核苷酸分型是最早基于PCR的基因分型方法之一。为了制定更有效的治疗策略，需要了解克尔曼沙赫市临床样本中MTB复合群样本中常见菌株的数量和鉴定信息。这是一项描述性横断面研究，研究对象为2021年12月至2022年6月期间转诊至克尔曼沙赫结核病中心的41例结核病样本患者，包括痰液、抽吸物、尿液等。首先，采用比例法将培养出的细菌对培养基的敏感性与异烟肼进行比较，并根据标准方案测定利福平。还记录了转诊至肺病控制中心的患者的人口统计学数据。下一步，使用标准方法进行寡核苷酸分型，将每种菌株模式记录为八进制代码，并与世界银行提供的有关寡核苷酸分型及其菌株的信息进行比较。对41例肺结核患者进行了寡核苷酸分型检测。鉴定出4种MTB菌株，包括H4、CAS、T1和H1。在通过寡核苷酸分型分离出的MTB复合群菌株中，H4菌株的频率最高，有16个样本（39%）。通过寡核苷酸分型分离出的菌株中频率最高的与H4菌株相关。可以得出结论，寡核苷酸分型具有成本效益高、简单、可重复和高度敏感的特点。
