Torkamani Ae
Department of Agriculture and Food systems (DAFS), School of Land and Environment, University of Melbourne, Melbourne, Vic 3010, Australia.
Iran J Microbiol. 2011 Sep;3(3):152-5.
Pulsed electric field (PEF) is a novel emerging technology which is believed to have the potential to substitute conventional thermal pasteurization (HTST). In the current study PEF was compared with HTST based on microbial inactivation and quality attributes.
Juice was prepared by extracting it from Semirum apples. They were chilled to 4°C over night. Then were divided into two lots, one was treated by PEF and the other by HTST. The treated juices were cultured on tryphtic soy broth (TSB) and results were recorded for 168 days. Quality changes were characterized by color and sensory test. Color changes were quantified using Hunter Lab equipment and equation. Sensory changes were evaluated by test panelists.
Using selective media E. Coli was enumerated, the total count of the organism was noticeably lower than PEF treated specimen and after 168. The count didn't reach the initial population. Whereas in PEF treated juice bacterial count bounced back to the initial count and exceeds. Results from Hunter Lab indicated a of 3.04 and 3.08 system for PEF and HTST treated juices. Sensory panel showed that PEF is superior to thermal treatment.
The study indicated HTST is more suitable based on food safety encounters. However PEF treated are closer to fresh juices based on quality factors. It can be concluded that PEF has the potential to become a suitable replacement to conventional process if improvements in design are applied.
脉冲电场(PEF)是一种新兴技术,被认为有潜力替代传统的高温瞬时杀菌(HTST)。在本研究中,基于微生物灭活和品质特性对脉冲电场与高温瞬时杀菌进行了比较。
从Semirum苹果中提取果汁制成样品。将其在夜间冷却至4°C。然后分为两批,一批用脉冲电场处理,另一批用高温瞬时杀菌处理。将处理后的果汁在胰蛋白胨大豆肉汤(TSB)中培养,并记录168天的结果。通过颜色和感官测试来表征品质变化。使用Hunter Lab设备和公式对颜色变化进行量化。由评审员对感官变化进行评估。
使用选择性培养基对大肠杆菌进行计数,该微生物的总数明显低于脉冲电场处理的样品,并且在168天后,计数未达到初始数量。而在脉冲电场处理的果汁中,细菌数量反弹至初始数量并超过该数量。Hunter Lab的结果表明,脉冲电场处理和高温瞬时杀菌处理的果汁的系统值分别为3.04和3.08。感官评定小组表明,脉冲电场处理优于热处理。
该研究表明,基于食品安全考虑,高温瞬时杀菌更合适。然而,基于品质因素,脉冲电场处理的果汁更接近新鲜果汁。可以得出结论,如果在设计上加以改进,脉冲电场有潜力成为传统工艺的合适替代方法。