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在亚适温条件下施用来自大豆根瘤菌的脂壳寡糖后，大豆全球基因表达的变化。

Changes in soybean global gene expression after application of lipo-chitooligosaccharide from Bradyrhizobium japonicum under sub-optimal temperature.

机构信息

Department of Plant Science, McGill University, Ste Anne de Bellevue, Quebec, Canada.

出版信息

PLoS One. 2012;7(2):e31571. doi: 10.1371/journal.pone.0031571. Epub 2012 Feb 13.

DOI:10.1371/journal.pone.0031571
PMID:22348109
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3278468/
Abstract

Lipo-chitooligosaccharides (LCOs), signal compounds produced by N(2)-fixing rhizobacteria after isoflavone induction, initiate nodule formation in host legumes. Given LCOs' structural similarity to pathogen-response-eliciting chitin oligomers, foliar application of LCOs was tested for ability to induce stress-related genes under optimal growth conditions. In order to study the effects of LCO foliar spray under stressed conditions, soybean (Glycine max) seedlings grown at optimal temperature were transferred to sub-optimal temperature. After a 5-day acclimation period, the first trifoliate leaves were sprayed with 10(-7) M LCO (NodBj-V (C(18:1), MeFuc)) purified from genistein-induced Bradyrhizobium japonicum culture, and harvested at 0 and 48 h following treatment. Microarray analysis was performed using Affymetrix GeneChip® Soybean Genome Arrays. Compared to the control at 48 h after LCO treatment, a total of 147 genes were differentially expressed as a result of LCO treatment, including a number of stress-related genes and transcription factors. In addition, during the 48 h time period following foliar spray application, over a thousand genes exhibited differential expression, including hundreds of those specific to the LCO-treated plants. Our results indicated that the dynamic soybean foliar transcriptome was highly responsive to LCO treatment. Quantitative real-time PCR (qPCR) validated the microarray data.

摘要

脂寡糖（LCOs）是固氮根瘤菌在诱导异黄酮后产生的信号化合物，能在宿主豆科植物中引发根瘤的形成。鉴于 LCOs 的结构与引发植物抗病反应的几丁寡糖类似，我们测试了叶面喷施 LCOs 能否在最佳生长条件下诱导与应激相关的基因。为了研究 LCO 叶面喷施在胁迫条件下的效果，我们将在最佳温度下生长的大豆（Glycine max）幼苗转移到亚最佳温度下。经过 5 天的适应期后，用 10(-7) M LCO（从诱导 genistein 的 Bradyrhizobium japonicum 培养物中纯化得到的 NodBj-V (C(18:1), MeFuc)）处理第一片三叶期叶片，在处理后 0 和 48 小时收获叶片。使用 Affymetrix GeneChip® Soybean Genome Arrays 进行微阵列分析。与 LCO 处理后 48 小时的对照相比，LCO 处理导致 147 个基因的表达发生差异，其中包括一些与应激相关的基因和转录因子。此外，在叶面喷施后的 48 小时内，有超过一千个基因表现出差异表达，其中包括数百个仅在 LCO 处理的植物中表达的基因。我们的结果表明，大豆叶片转录组对 LCO 处理具有高度的响应性。实时定量 PCR（qPCR）验证了微阵列数据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9461/3278468/c8a7a12cb847/pone.0031571.g013.jpg
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