Department of Hematology, All India Institute of Medical Sciences, New Delhi.
Mediterr J Hematol Infect Dis. 2012;4(1):e2012006. doi: 10.4084/MJHID.2012.006. Epub 2012 Jan 21.
High performance liquid chromatography (HPLC) and electrophoresis are commonly used to diagnose various hemoglobinopathies. However, insufficient information about the transfusion history can lead to unexpected and confusing results. We are reporting a case of Juvenile myelomonocytic leukemia (JMML) in which HbHPLC was done to quantify fetal hemoglobin (HbF). The chromatogram showed elevated HbF along with a peak in the HbD window. A transfusion acquired peak was suspected based on the unexpectedly low percentage of HbD and was subsequently confirmed using parental HbHPLC.
高效液相色谱法（HPLC）和电泳常用于诊断各种血红蛋白病。然而，由于缺乏有关输血史的信息，可能导致出现意外和令人困惑的结果。我们报告了一例幼年型粒单核细胞白血病（JMML）的病例，该患者进行 HbHPLC 以定量胎儿血红蛋白（HbF）。色谱图显示 HbF 升高，同时在 HbD 窗口出现一个峰。根据 HbD 的百分比异常低，怀疑存在输血获得的峰值，随后使用父母的 HbHPLC 进行了确认。
